Quick and mask-less cell two-dimensional graphical manufacturing method

Abstract

The invention relates to a quick and mask-less cell two-dimensional graphical manufacturing method. According to the method, a technology of inducing a hydrogel by ultraviolet light to generate a polymerization reaction is adopted to form the hydrogel with controllable graphic on a glass surface and for controlling cell growth. A photoinitiator absorbs light spot ultraviolet light to generate free radicals so as to trigger the free radicals of a PEGDA monomer to generate a cross-linking reaction to realize conversion from a liquid state to a solid state; by adoption of the hydrogel micro graphical manufacturing method, any physical template is not needed, and a corresponding binary graphic can be designed through a computer according to the structure of the needed hydrogel; and the graphic is reflected to a glass substrate through ultraviolet light to obtain the needed hydrogel two-dimensional graphic structure of the PEGDA. By adoption of the method, the cell growth position and the cell growth graphic can be accurately controlled, the growth shape and dimension of cells can be regulated by controlling the cured shape and dimensions of the PEGDA hydrogel, so as to make research on influence of external physical environment of cells to cell growth.

Description

technical field [0001] The invention relates to a technology for quickly producing two-dimensional cell graphics, in particular to a fast maskless two-dimensional cell graphics production method based on a digital micromirror array. Background technique [0002] Biology is considered to be the most promising subject in the 21st century, not only because biology currently has a large number of problems and challenges that need to be solved urgently, but more importantly, it is closely related to human life such as population, disease, health and other issues . Cell biology is one of the important basic disciplines of biology. It is a discipline that studies the structure, function and various laws of life of cells at the three levels of microscopic, submicroscopic and molecular levels. Cells play a vital role in biology. As individuals, cells can grow, proliferate, and die independently. As a whole, cells can cooperate together to form tissues and organs with specific functi...

AI Technical Summary

Problems solved by technology

Most of the existing patterning methods are top-down processing methods, such as photolithography, but these methods are costly and require high environmental requirements

Other methods are bottom-up patterning methods, mainly including soft lithography, scanning probe processing and nanoimprinting. Among these methods, the preparation process and operation are very complicated and time-consuming. Some also require complex chemical modifications

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