Rice ARP (Auxin Response Protein) gene cloning and application
An auxin and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of lack of, restricting the high-level development of molecular breeding and molecular design breeding, etc. Effect
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[0011] The implementation of the present invention will be described in further detail below. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.
[0012] A rice auxin response protein gene ARP, the gene is located on chromosome 4 of the rice genome, 3.8 kb away from the 4,5,7-trihydroxyflavanone dioxygenase gene, the gene has no intron, and the size is 462 base sequence, its gene sequence is SQ1.
[0013] An application of the rice auxin response protein gene ARP, the application is to apply the rice auxin response protein gene ARP to the fields of rice transgenic breeding, molecular design breeding and rice dwarf breeding.
[0014] The discovery and verification process of the rice auxin-responsive protein gene ARP described above includes the following steps:
[0015] (1) Preparation of rice transformed receptors
[0016] The mature seeds of rice variety Nipponbare are shelled, soaked in 70% alcohol...
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