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Acellular cornea stroma lens and preparation method thereof

A corneal stroma and decellularization technology, applied in the field of tissue engineering, can solve the problems of unstable performance, poor transparency, and low safety of corneal lenses, and achieve the effects of improving mechanical strength, maintaining transparency, and avoiding immune rejection.

Inactive Publication Date: 2017-10-10
GUANGZHOU SUN SHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The first object of the present invention is to provide a preparation method of decellularized corneal stroma lens, and the second object of the present invention is to provide decellularized corneal stroma lens to alleviate the low safety and transparency of corneal lens existing in the prior art. Technical issues with poor and inconsistent performance

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  • Acellular cornea stroma lens and preparation method thereof
  • Acellular cornea stroma lens and preparation method thereof
  • Acellular cornea stroma lens and preparation method thereof

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preparation example Construction

[0033] The invention provides a method for preparing a decellularized corneal stroma lens.

[0034] Wherein, the 0.1%-3% TritonX-100 involved in the method of cell lysis can be, for example, but not limited to 0.1% TritonX-100, 0.3% TritonX-100, 0.5% TritonX-100, 0.8% TritonX-100, 1 %TritonX-100, 1.5% TritonX-100, 2% TritonX-100, 2.5% TritonX-100 or 3% TritonX-100.

[0035] Among them, the crosslinking agent involved in the crosslinking method can be, but not limited to, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, or N-hydroxysulfosuccinimide , or genipin, or glutaraldehyde.

[0036] Wherein, the mass ratio of the cross-linking agent used in the cross-linking method to the corneal stromal lens is 1:1-1:15, and the mass ratio can be, but not limited to, 1:1, 1:2, 1 :3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:10, 1:11, 1:12, 1:13, 1:14 Or 1:15.

[0037] Among them, the concentration involved in the disinfection method is 0.01-0.1 mg / ml penicillin and 0.05-0.5 mg / ml streptom...

Embodiment 1

[0044] Embodiment 1 Preparation method of decellularized corneal stroma lens

[0045] Step (a), disinfection: Soak the corneal stromal lens with the accurate thickness of cells obtained by the full femtosecond laser technology in physiological saline containing 0.01 mg / ml penicillin and 0.1 mg / ml streptomycin 3h, then rinse with 0.9% saline;

[0046] Step (b), cell lysis: immerse in 0.9% normal saline containing 0.5% TritonX-100 for 24 hours, then rinse with 0.9% normal saline for 96 hours;

[0047] Step (c), cross-linking: Soak in 0.9% physiological saline containing a cross-linking agent, then rinse with 0.9% physiological saline for 24 hours, and the cross-linking agent is 1-(3-dimethylaminopropyl)-3-ethane Carbodiimide (EDC); the mass ratio of the amount of cross-linking agent to the corneal stroma lens processed through cell lysis is 1:5;

[0048] Step (d), sterilizing: irradiating the crosslinked corneal lens with gamma rays, and the irradiation dose is 25kGy;

[0049...

Embodiment 2

[0056] Embodiment 2 Preparation method of decellularized corneal stroma lens

[0057] Step (a), disinfection: Soak the corneal stromal lens with the accurate thickness of the cells obtained by the full femtosecond laser technology in physiological saline containing a concentration of 0.05 mg / ml penicillin and a concentration of 0.2 mg / ml streptomycin 2h, and then rinse with 0.9% saline;

[0058] Step (b), cell lysis: immerse in 0.9% normal saline containing 0.3% TritonX-100 for 48 hours, then rinse with 0.9% normal saline for 96 hours;

[0059] Step (c), cross-linking: Soak in 0.9% physiological saline containing cross-linking agent, then rinse with 0.9% physiological saline for 24h, cross-linking agent N-hydroxysulfosuccinimide (NHS); cross-linking agent The mass ratio of the usage amount to the corneal stroma lens processed by cell lysis is 1:9;

[0060] Step (d), sterilizing: irradiating the crosslinked corneal lens with gamma rays, and the irradiation dose is 25kGy;

[...

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Abstract

The invention provides an acellular cornea stroma lens and a preparation method thereof, and relates to the technical field of tissue engineering. By means of the preparation method of the acellular cornea stroma lens, cellular constituents in stromata can be effectively removed, the immunogenicity of the cornea stroma lens is reduced, the mechanical strength of the cornea stroma lens is improved, and moreover, the original morphology and the transparency of the cornea stromata can be effectively kept. The prepared acellular cornea stroma lens has the advantages of being higher in safety, better in transparency and more stable in performance; the lens can be used as a scaffold for the proliferation and growth of cornea stroma cells, and finally, the lens and self-body corneas are fused together and serves as a permanent implant lens; besides, the phenomena of immunological rejection and the like can be avoided.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a decellularized corneal stroma lens and a preparation method thereof. Background technique [0002] Hyperopia is a state of refraction behind the retina after parallel light rays enter the eye. When the refractive power of the eyeball is insufficient or the axial length of the eye is insufficient, hyperopia occurs. As a kind of ophthalmic disease, hyperopia greatly affects people's quality of life, especially as the age increases, the probability of hyperopia will also increase. [0003] Hyperopia is a common ametropic eye disease in clinical practice, and the correction of high hypermetropia has always been a difficult problem in refractive therapy. The current methods of hyperopia correction treatment mainly include glasses, contact lenses or refractive surgery. [0004] Presbyopia is a physiological phenomenon, not a pathological state, nor a refractive error. It...

Claims

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Application Information

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IPC IPC(8): A61F2/16A61L27/36
Inventor 梁丽金杨习锋曾晨光余克明庄菁
Owner GUANGZHOU SUN SHING BIOTECH CO LTD
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