Synchronous separation-extraction technique for intestinal mucous byproduct

Abstract

The invention provides a synchronous separation-extraction technique for an intestinal mucous byproduct, relating to the technical field of biological pharmacy and feed production. The synchronous separation-extraction technique comprises the following steps: firstly extracting heparin sodium through salt decomposition, separating heparin from proteins, and extracting crude product heparin; and activating proteins produced during the production by virtue of fresh pancreas, and carrying out enzymolysis, so as to produce protein peptide. The product contains rich small peptide easily digested and absorbed by livestock and is a novel animal derived feed protein rich in short peptide chains and balanced amino acids. By utilizing the product, the production performance and health level of animals, particularly young animals, can be enhanced; and amino acids existing in a short peptide manner and free amino acids have relatively high biological activities and can be taken as protein feed sources of multiple animals.

Description

technical field [0001] The invention relates to the technical field of biopharmaceutical and feed production. Background technique [0002] At present, the vast majority of manufacturers that extract heparin sodium from pig small intestinal mucosa use the following method to produce heparin sodium: add 4 times the amount of saline with a concentration of 3% to 4% to the intestinal mucosa, and adjust the pH to 7.5 to 8.5 with sodium hydroxide solution. Add enzyme at the ratio of 3g enzyme to each small intestine, and raise the temperature to 55°C-58°C, keep warm for 2 hours for enzymolysis, then raise the temperature of enzymolyzed mucous membrane to 80-85°C, keep warm for 20 minutes, filter and cool the filtrate To 55°C, add the resin into the enzymolysis solution to absorb heparin sodium according to the ratio of 15-20g resin per small intestine, and absorb for 8 hours. Filter and discharge the filtrate. Collect the resin and elute twice with 20 Baume degree saline accordi...

AI Technical Summary

Problems solved by technology

[0003] Using the above process to produce 1 kg of intestinal membrane will produce about 4 kg of sewage, which contains COD20000-25000, which is very troublesome and expensive to deal with. Most manufacturers cannot recycle it and have to discharge it as waste water, which wastes a lot of protein resources. , and seriously pollute the environment, restricting the development of this product