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A SNP and detection kit for early assessment of the risk of esophageal cancer

A kit and technology for esophageal cancer, applied in the field of SNP and detection kits for early assessment of the risk of esophageal cancer, can solve the problems that it is not suitable for frequent use

Active Publication Date: 2020-09-25
CHIFENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Although X-ray barium meal and gastroscopy are commonly used to detect esophageal cancer, such tests are invasive and not suitable for frequent use

Method used

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  • A SNP and detection kit for early assessment of the risk of esophageal cancer
  • A SNP and detection kit for early assessment of the risk of esophageal cancer
  • A SNP and detection kit for early assessment of the risk of esophageal cancer

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Experimental program
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Embodiment Construction

[0017] We collected peripheral blood from 160 patients with esophageal cancer and 204 normal controls. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0018] The steps of the allele-specific PCR method are as follows.

[0019] (1) There are two tubes, A and B. The difference between the two tubes is that the upstream wild-type primer P1 is added to the A tube, and the upstream mutant primer P2 is added to the B tube, and the rest are the same.

[0020] (2) PCR reaction system: See the table below for the ingredients in tubes A and B.

[0021] Tube A A tube B tube Sterilized water: 5 μl Sterilized water: 5 μl PCR mix: 10 μl PCR mix: 10 μl DNA template: 1μl (100-150ng / μl) DNA template: 1μl (100-150ng / μl) P1: 1 μl (10 μM) P2: 1 μl (10 μM) P3: 1 μl (10 μM) P3: 1 μl (10 μM) P4: 1 μl (10 μM) P4: 1 μl (10 μM) P5: 1 μl (10 μM) P5: 1 μl (10 μM) Total: ...

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Abstract

The invention discloses a detection kit for single nucleotide polymorphism sites in high correlation to esophageal squamous carcinoma incidence. The kit comprises two forward allele specific primers aiming at the 5-lipoxygenase E254K site design, one reverse primer, an internal reference primer, PCR (Polymerase Chain Reaction) mix and sterile water. The kit adopted by the invention is capable of rapidly detecting 5-LO E254K sites in high correlation to esophageal squamous carcinoma incidence, and has the characteristics of simplicity, economic efficiency, rapidness and the like compared with the classical sequencing and enzyme digestion methods, and an allele specific PCR method based on the kit disclosed by the invention is a method which is mature and reliable and can be widely used in primary hospitals.

Description

technical field [0001] The present invention relates to human peripheral blood genomic DNA and uses allele-specific PCR method to detect 5-lipoxygenase (5-lipoxygenase, 5-LO) c. A kit for early assessment of the risk of esophageal cancer. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yanting in Sichuan Province, Chaoshan in Guangdong Province, and Huai'an in Jiangsu Province were established as the six high-incidence areas for esophageal cancer in China. The Chifeng area of ​​Inner Mongolia is an area with a high incidence of esophageal cancer in northern my country. Our preliminary epidemiological investigation results show that the incidence ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156C12Q2600/166
Inventor 白春英苏志勇瑞云史铁伟许丽艳李恩民赖青金品辛王亚奇张佳乐孙斌王治国
Owner CHIFENG UNIV