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A multi -group sample tissue baptism method

A sample and tissue technology, which is applied in the field of co-embedding of multiple samples and tissues, can solve the problems of reducing observation and achieve the effect of eliminating slices

Active Publication Date: 2019-10-25
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, paraffin sections can only embed one tissue cell after sectioning, which reduces the speed and efficiency of observing and studying tissue lesions.

Method used

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  • A multi -group sample tissue baptism method
  • A multi -group sample tissue baptism method
  • A multi -group sample tissue baptism method

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Experimental program
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Embodiment Construction

[0027] A method for co-embedding multiple groups of sample tissues, comprising the steps of:

[0028] 1 Preparation of sample tissue-embedded framework

[0029] 1.1 Take the skin tissue (2cm×1cm) of the scrotum of male animals of the same species, soak it in 4% paraformaldehyde and fix it for more than 24 hours.

[0030] 1.2 Fix the above-mentioned treated animal skin, carry out routine dehydration, fix and soak in wax, immediately flatten the animal skin and fix it on a foam board after waxing, and cool it in an environment of 0°C.

[0031] 1.3 Trim the skin block, use a scalpel to trim the skin block into: 0.5cm×1cm, rectangular (such as figure 1 shown).

[0032] 1.4 At a distance of 5mm from one wide side of the rectangular skin block, make a 0.25cm long vertical incision on the long side, and slightly expand the width of the incision, so that the long side on the other side of the incision becomes 7mm. (Such as figure 2 shown).

[0033] 1.5 Select the skin block acco...

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Abstract

The invention relates to a method for co-embedding tissues of multiple sets of samples. The method comprises the following steps: taking fresh male animal scrotum skin tissues which are homologous to to-be-detected samples; cutting into skin blocks in preset size; removing all the subcutaneous tissues and fat; soaking in 4-6% paraformaldehyde solution for more than 24 hours; conventionally dewatering, waxing and fixing on a support plate; cooling under the environment at 0 DEG C; after cooling, cutting the waxed animal skin into the animal skin blocks in preset size and shape according to the requirement under the environment at 0 DEG C; vertically arranging the animal skin blocks into a plurality of embedded areas at certain angles; respectively setting the tissues of multiple samples in the sample tissue embedding areas and then using paraffin for sealing into an integrated structure; absorbing sample tissue membranes to a polylysine coated glass slide, baking the slice for 2 hours in a drying oven at 60 DEG C and conventionally de-waxing. According to the method provided by the invention, the detection and analysis precision for the sample tissues can be increased, the error caused by manual operation can be reduced and the working efficiency can be increased.

Description

technical field [0001] The invention relates to a paraffin body for embedding biological tissues, in particular to a co-embedding method for multiple groups of sample tissues, which can simultaneously analyze, observe, detect or test multiple groups of sample biological tissues, improve work efficiency, reduce human error. Background technique [0002] Paraffin embedding technology is one of the early tissue processing technologies of paraffin section technology. Paraffin section technology has a history of more than 300 years and is the most widely used technique in routine histology. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science, and has been widely used in many other disciplines such as biology. research in the field. Usually, the diseased tissue is embedded in paraffin bloc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/36G01N1/30
CPCG01N1/30G01N1/36G01N2001/368
Inventor 马贤德吴景东金岩王哲张威左韬关洪全
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE