Method for evaluating lily cold resistance and screening winter-resisting variety and application

Abstract

The invention discloses a method for evaluating lily cold resistance and screening winter-resisting variety and an application and belongs to an agricultural efficient production technology. The method comprises the following steps: by taking the first mature leaf of the second round below the growth point of the lily plant as a raw material, cutting the mature leaf into the leaf blocks in certain size and pre-treating the leaf blocks; utilizing a low-temperature circulating instrument to treat the leaf blocks and then measuring the relative conductivity; and lastly, utilizing statistical software to calculate lethal dose-50 temperature (LT50) of various lily varieties, evaluating the cold resistance of the lily varieties according to the lethal dose-50 temperature and screening the winter-resisting variety of lily. Compared with the traditional method for evaluating the cold resistance by taking lily seed ball or tissue culture seedling as a raw material, the method has the advantages that the evaluation result of the cold resistance of lily can reflect the practical cold resistance of lily in the field and the method is more representative than the traditional method.

Description

technical field [0001] The present invention mainly relates to the field of lily physiology, and in particular to a method for evaluating lily cold resistance and screening cold-resistant varieties and its application. The method mainly uses low-temperature treatment on the leaves in the vegetative growth period of lily, and obtains the relative electrical conductivity by measuring the electrical conductivity of the leaves. Fit the Logistic regression equation, and then calculate the semi-lethal temperature (LT) of each variety of lily. 50 ), evaluate the cold tolerance of lily varieties, and screen out cold tolerance lily varieties. technical background [0002] Lily is a perennial flower bulb belonging to the family Liliaceae. Commonly said lily refers to the general term of various and varieties of Lilium (Lilium spp.) plants. Lily has huge flowers, bright colors, various poses, and pleasant fragrance. As an important ornamental plant, it can be used not only as cut flo...

AI Technical Summary

Problems solved by technology

At present, in the selection of cold resistance of lily varieties, it is mainly through low-temperature treatment of lily bulbs and lily tissue culture seedlings, and by measuring the electrical conductivity, MDA, POD, and protective enzyme activities under low temperature stress to screen lily cold-resistant varieties. There are some disadvantages in these methods, such as bulb low-temperature treatment and screening in the screening process, the processing of each variety will consume a large amount of bulbs, and when there are many varieties, it is time-consuming and labor-intensive; high, and the growth cycle of tissue culture seedlings is long, and it is time-consuming and laborious to start from tissue culture; in addition, the cold resistance evaluation results of lily bulbs and tissue culture seedlings are often quite different from the cold resistance of lily plants in the field.

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