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Solid phase microextraction method of unsaturated fatty acid

An unsaturated fatty acid and extraction technology, applied in the field of analytical chemistry, can solve problems such as indistinguishability, qualitative and quantitative analysis effects, etc., and achieve the effect of simple method, improved separation degree and ingenious process

Active Publication Date: 2017-10-24
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main difficulty in TFA detection is that the structure of TFA is very similar to its cis-isomer (Cis-fatty acid, CFA) that exists in a large number of foods, making it difficult to distinguish them. cause greater impact

Method used

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  • Solid phase microextraction method of unsaturated fatty acid
  • Solid phase microextraction method of unsaturated fatty acid
  • Solid phase microextraction method of unsaturated fatty acid

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preparation example Construction

[0032] 1. Preparation of mucopolysaccharide functionalized monolithic column:

[0033] 1) Cleaning of PTFE tube empty column: Connect a PTFE tube with an inner diameter of 750 μm to a liquid phase pump, wash it with chromatographically pure methanol at a flow rate of 0.5 mL / min for 10 min to remove residual organic impurities on the inner wall of the tube, and then pass nitrogen gas. and dried in an oven at 60 °C for 10 min.

[0034]2) Rapid polycondensation in the tube: Mix 8 mg sodium hyaluronate, 550 mg 1 g / mL urea solution, 450 mg formaldehyde solution, and 100 mg 0.2 mol / L hydrochloric acid solution evenly, and oscillate rapidly for 1 to 2 min, and then dissolve the mixture Quickly fill the cleaned and dried PTFE tube (or PEEK tube), seal both ends and immerse in a water bath at 70 °C for 10 min at a constant temperature; after the reaction is completed, use water as the mobile phase and rinse the PTFE tube on the liquid chromatography pump Keep the monolithic column for...

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Abstract

The invention discloses a solid phase microextraction method of unsaturated fatty acid. A silver nanoparticle immobilized monolithic column is used as a solid phase microextraction monolithic column, and the solid phase microextraction method of unsaturated fatty acid is established in combination with an in-tube solid phase microextraction-high performance liquid chromatography system. The unsaturated fatty acid is enriched under the interaction of silver nanoparticles on the surface of the monolithic column and carbon-carbon double bonds of the unsaturated fatty acid; different isomers of the unsaturated fatty acid are sequentially eluted under different acting forces between the silver nanoparticles and the isomers of the unsaturated fatty acid. The method is simple, the process is ingenious, required instruments have higher popularization degree, the method is easy to popularize, efficient enrichment of the unsaturated fatty acid and sequential elution of different cis-trans-isomers can be realized, and the efficient detection requirement of trans-fatty acid can be met.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a solid-phase microextraction method for unsaturated fatty acids. Background technique [0002] Unsaturated fatty acids are the main components of edible vegetable oils. The carbon-carbon double bonds in unsaturated fatty acids exist in two forms: cis and trans: unsaturated fatty acids formed by cis bonds are liquid at room temperature, and trans bonds The unsaturated fatty acids formed are solid at room temperature. In the modern food industry, in order to prevent oil from deteriorating and improve the flavor of processed food, people partially hydrogenate vegetable oil to obtain partially hydrogenated vegetable oil. A large number of unsaturated fatty acids contained in partially hydrogenated vegetable oils are trans fatty acids (Trans-fatty acid, TFA) that can be solid at room temperature. Therefore, partially hydrogenated oil has the advantages of high temper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/60
CPCG01N30/02G01N30/06G01N30/6052G01N2030/062
Inventor 王家斌姜楠李文邦张其清
Owner FUZHOU UNIV
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