A method for visible identification of chiral sulfenamides
A sulfenamide and chiral technology, applied in the field of chiral recognition, can solve the problems of complex post-processing and cumbersome testing procedures
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Embodiment 1
[0079] Example 1. Recognition of Chiral tert-Butylsulfinamide by Supramolecular Polymerization of Diacetylene Glutamic Acid Derivatives - Using Vesicle System for Recognition
[0080] Weigh 5 mg of the synthesized diacetylenyl glutamic acid derivative into a 100 ml round flask, and add 50 ml of dichloromethane to dissolve it.
[0081] After being completely dissolved, place it on a rotary evaporator for rotary evaporation until the solvent evaporates completely.
[0082] Add 50ml of ultrapure water, and ultrasonically shake at 70°C for 20 minutes to obtain the vesicle structure.
[0083] Prepare R-type tert-butylsulfinamide aqueous solution and S-type tert-butylsulfinamide aqueous solution with different concentrations. Concentrations are 0M, 0.02M, 0.1M, 0.2M, 0.5M, 1M, 1.5M, 2M
[0084] Take 1ml of the vesicle-containing aqueous solution, add it to a 5ml sample bottle, and irradiate it with 254nm ultraviolet light to turn into a blue solution.
[0085] Add 50ul of differe...
Embodiment 2
[0089] Example 2. Recognition of Chiral tert-Butylsulfinamide by Supramolecular Polymerization of Diacetylene Glutamic Acid Derivatives—Recognition Using Gel System
[0090] Weigh 5 mg of the synthesized diacetylenyl glutamic acid derivative into a 3 ml sample bottle, and add 250 ul of methanol for dissolution.
[0091] After it is completely dissolved, add 250ul of water, some uneven precipitation occurs, heat slightly until it is completely dissolved, and let it stand at room temperature.
[0092] After standing at room temperature for 2 hours, a white translucent gel formed.
[0093] The gel was irradiated with 254nm ultraviolet light to turn into a blue gel.
[0094] Add R-type and S-type tert-butylsulfinamide respectively, shake and mix evenly, and add until the final concentration of R-type tert-butylsulfinamide and S-type tert-butylsulfinamide is 2M.
[0095] By visual observation, it can be seen that the gel added with S-type tert-butyl sulfinamide turns from blue to...
Embodiment 3
[0098] Example 3. Recognition of chiral p-toluenesulfinamide by supramolecular polymerization of diacetylenyl glutamic acid derivatives—recognition by gel system
[0099] The synthesized and good diacetylenic glutamic acid derivatives were prepared as 4mg / ml methanol solution.
[0100] Take 100ul of the prepared methanol solution and add it to a 1mm cuvette.
[0101] Prepare R-type p-toluenesulfinamide methanol solution and S-type p-toluenesulfinamide methanol solution with a concentration of 2.5M.
[0102] Add 25ul of prepared R-type and S-type p-toluenesulfinamide methanol solutions to two cuvettes, shake gently and mix well.
[0103] Then add 275ul of high-purity water to the cuvette, shake gently and let it stand for 2 hours to form a translucent gel.
[0104] Both gels were irradiated with UV light at 254 nm.
[0105] By visual observation, it can be seen that the gel added with S-type p-toluenesulfinamide directly turns purple, while the gel added with R-type p-toluen...
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