Two-channel animal nerve cell signal recording and synchronous irritation system

Abstract

The invention belongs to the fields of biology, neuroscience, medicine and the like, and particularly relates to the field of photogenetics technology application, in particular to a two-channel animal nerve cell signal recording and synchronous irritation system. The photogenetics excitation and inhibition experiments can be realized at the same time in the process of recording the activity of animal nerve cells doing free activities. The system starts from the recording and the illumination of the relationship between target nerve cell self-activating signals and target behaviors through nerve cell self-activating signals; then, the verification is respectively performed from positive and negative points of view through nerve cell excitation experiments and inhibition experiments; and the scientificity and the accuracy of the experiment results are fully ensured. One optical fiber can be used for realizing the recording and stimulation functions; the process of switching recording and stimulation devices in the experiment process is avoided; the experiment time is reduced; and the experiment steps are simplified, so that the whole experiment period is greatly shortened.

Description

technical field [0001] The invention belongs to the fields of biology, neuroscience, medicine, etc., and particularly relates to the application field of optogenetics technology, in particular to a dual-channel animal neuron signal recording and synchronous stimulation system. Background technique [0002] The human brain is composed of nearly 100 billion nerve cells. Neurons form a network through synapses, communicate with each other, transmit information, and form sensory, motor and other systems. Each system can be divided into several subsystems, such as vision in the senses. , Smell, hearing, taste and body surface touch, etc., the implementation of its functions depends on the precise connection between different types of cells in different parts of the nervous system. Neural circuits are the bridge between molecular and cellular functions and overall behavioral functions. The study of specific functional neural circuits is conducive to understanding the formation and...

AI Technical Summary

Problems solved by technology

From the perspective of genetics technology, a variety of light-sensitive channels have been developed, which can be conveniently expressed on cultured cells or in living animals; long-term real-time monitoring of the neuron activity of each nucleus in the brain of freely moving animals is an important way to explore brain function and find One of the important methods for the pathogenesis of nerve-related diseases. Traditionally, electrophysiological methods are mainly used, but the experimental steps are com

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