In-vivo enrichment device of circulating free DNA

An enrichment and bulk technology, applied in the field of medical devices, can solve the problems of low efficiency and low quality in vitro capture, and achieve excellent biocompatibility, reduce phlebitis, and excellent mechanical properties

Active Publication Date: 2017-11-10
HUADONG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an in vivo enrichment device for circulating free DNA, thereby solving the problem of low efficiency and low quality of in vitro capture of circulating free DNA in the prior art

Method used

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  • In-vivo enrichment device of circulating free DNA
  • In-vivo enrichment device of circulating free DNA
  • In-vivo enrichment device of circulating free DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as figure 1 Shown is an in vivo enrichment device 10 according to a preferred embodiment of the present invention. The device includes: a catheter body 1 that can reach the central vein through peripheral venous puncture, a plurality of sieve holes 2 are distributed on the tube wall of the catheter body 1 , and magnetic beads 3 that specifically bind ccfDNA are filled in the catheter body 1 . The magnetic beads 3 can be commercially available magnetic beads for nucleic acid extraction. The aperture of the mesh 2 is smaller than that of the magnetic beads 3 to prevent the magnetic beads 3 from leaking out of the catheter body 1 . From figure 1 It can be seen from the figure that the catheter body 1 has a uniform diameter along its length. The front end 4 of the catheter body 1 is a blind end to prevent the magnetic beads from leaking out.

[0038] Preferably, about 10 mm of the front end of the catheter body 1 is passivated to reduce damage to blood vessels and ...

Embodiment 2

[0044] Such as figure 2 Shown is an in vivo enrichment device 20 according to another preferred embodiment of the present invention. The structure of the device 20 is substantially similar to that of the device 10 of the previous embodiment, the only difference is that the catheter body 1 has an uneven diameter along its length. According to this particularly preferred embodiment, the catheter body 1 has along its length a plurality of bulbous projections 12 spaced apart from each other by a cylindrical section 11 , the tube diameter of which is always greater than that of the cylindrical section 11 . This structure is also called "spherical expansion type". The advantage of this "spherical expansion type" is that it can increase the volume of blood in contact with the magnetic beads 3. If the clinical stage is earlier and the vessel diameter is appropriate, this "spherical expansion type" is theoretically preferred for high efficiency and high efficiency. Texture collectio...

Embodiment 3

[0047] According to this embodiment, the present invention also provides an optional operating procedure for the above-mentioned in vivo enrichment device 10, specifically as follows:

[0048] 1) The device should be used by medically professionally trained personnel, always observing routine hygienic requirements.

[0049] 2) Position the patient so that the patient's limb is at 90 degrees to the body.

[0050] 3) Place a waterproof pad or a disposable pad under the punctured limb, and place a tourniquet.

[0051] 4) Select the puncture site. The principle of vein selection is as follows: the basilic vein is the first choice, followed by the median vein, and then the cephalic vein.

[0052] 5) From the pre-puncture point along the vein to the right sternoclavicular joint, subtract 2-5cm from the measured value to get the catheter insertion length.

[0053] 6) Wear a sterile isolation gown (assisted by an assistant) and sterile gloves.

[0054] 7) Lay out the sterile table:...

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Abstract

The invention provides an in-vivo enrichment device of circulating free DNA. The device comprises a conduit body which can reach a central vein through peripheral vein puncture, wherein multiple sieve holes are distributed in the wall of the conduit body; the conduit body is filled with magnetic beads which have specific binding with the circulating free DNA; and the front end of the conduit body is a blind end. According to the in-vivo enrichment device provided by the invention, the conduit body is filled with the magnetic beads which have specific binding with the circulating free DNA, and the sieve holes are formed in the conduit wall of the conduit body, so ccfDNA in blood is continuously bound specifically to the magnetic beads inside the conduit body, so efficient and high-quality capture of the ccfDNA in bloods can be achieved. The device provided by the invention is characterized by in-vivo use, efficient capture and clinical portable application; molecule gene diagnosis information based on ccfDNA can be provided for earlier tumor diagnosis, targeted therapy, relapse transfer detection, treatment effect assessment and so forth, and application prospect is very wide.

Description

technical field [0001] The invention relates to the field of medical devices, and more particularly to an in vivo enrichment device for circulating free DNA. Background technique [0002] With the continuous improvement of the requirements for precise diagnosis and treatment of malignant tumors, liquid biopsy technology represented by circulating tumor cells (CTC) and circulating cell-free DNA (ccfDNA) has become a research hotspot at home and abroad in recent years. Circulating cell-free DNA (ccfDNA) refers to DNA molecules that are in a free state outside the cells, in human blood (serum or plasma), synovial fluid, cerebrospinal fluid, urine, prostatic fluid, lymph fluid, saliva, It can be detected in body fluids such as tracheal lavage fluid. ccfDNA is a kind of extracellular DNA in a cell-free state, mainly composed of single-stranded or double-stranded DNA and a mixture of the two, and exists in two forms of DNA-protein complexes or free DNA. [0003] In healthy peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/02C12N5/09
CPCA61B10/02C12N5/0693
Inventor 焦玉新郑向鹏任艳萍保志军
Owner HUADONG HOSPITAL
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