Method for promoting growth of Haematococcus Pluvialis and accumylation of astaxanthin by using plant hormones
A technology of Haematococcus pluvialis and phytohormones, applied in the directions of adding compounds to stimulate growth, microorganism-based methods, biochemical equipment and methods, etc. Accumulation of cyanin, low production cost, and the effect of increasing economic benefits
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Embodiment 1
[0025] A method for promoting the growth of Haematococcus pluvialis and the accumulation of astaxanthin by using plant hormones, comprising the following steps:
[0026] 1. Fully dissolve naphthaleneacetic acid with 95% ethanol, and dilute it into pure water to prepare a 1g / L mother liquor;
[0027] 2. Prepare the growth medium of Haematococcus pluvialis, and add the mother liquor of naphthaleneacetic acid to the medium, and the concentration of naphthaleneacetic acid is 0mg / L, 2mg / L;
[0028] 3. Take the algae liquid in the logarithmic phase and inoculate the culture medium at a ratio of 1:5;
[0029] 4. Place the above-mentioned algae liquid at 24°C under 1000-4000lx light for ventilation and culture, measure the biomass every two days, and cultivate continuously for 8 days;
[0030] 5. Through data analysis, it is obtained that in the medium added with 2mg / L naphthaleneacetic acid, the growth rate of the algae fluid is accelerated, and after 8 days, the biomass is increase...
Embodiment 2
[0032] A method utilizing plant hormones to promote the growth of Haematococcus pluvialis, comprising the following steps:
[0033] 1. Fully dissolve naphthaleneacetic acid with 95% ethanol, and dilute it into pure water to prepare a 1g / L mother liquor;
[0034] 2. Prepare the growth medium of Haematococcus pluvialis, and add the mother liquor of naphthaleneacetic acid to the medium, and the concentration of naphthaleneacetic acid is 0 mg / L, 2 mg / L;
[0035] 3. Take the algae liquid in the logarithmic phase and inoculate the culture medium at a ratio of 1:10;
[0036] 4. Place the above-mentioned algae liquid at 24°C under 1000-4000lx light for ventilation and culture, measure the biomass every two days, and cultivate continuously for 10 days;
[0037] 5. Through data analysis, it is obtained that in the medium added with 2mg / L naphthaleneacetic acid, the growth rate of the algae fluid is accelerated, and after 10 days, the biomass is increased by 10% compared with the algae ...
Embodiment 3
[0039] A method utilizing plant hormones to promote the growth of Haematococcus pluvialis, comprising the following steps:
[0040] 1. Fully dissolve naphthaleneacetic acid with 95% ethanol, and dilute it into pure water to prepare a 1g / L mother liquor;
[0041] 2. Prepare microalgae growth medium, and add naphthaleneacetic acid mother liquor to the medium, and the concentration of naphthaleneacetic acid is 0 mg / L, 1 mg / L, 2mg / L and 3mg / L;
[0042] 3. Take the algae liquid in the logarithmic phase and inoculate the culture medium at a ratio of 1:5;
[0043] 4. Place the above-mentioned algae liquid at 24°C under 1000-4000lx light for ventilation and culture, measure the biomass every two days, and cultivate continuously for 8 days;
[0044] 5. Through data analysis, it is obtained that in the medium added with naphthaleneacetic acid, the growth rate of the algae liquid is significantly accelerated, and the biomass of the algae liquid in the medium with 3mg / L naphthaleneacetic...
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