Preparation method of compound bacterium agent for degrading phosphorus
A technology of composite bacteria and bacterial agents, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem that the effect of nitrogen and phosphorus removal cannot meet the satisfactory requirements, and the technical problems of phosphorus and nitrogen removal , can not meet the discharge standards and other problems, to shorten the initial commissioning time, provide the effect of denitrification and dephosphorization, and rapid denitrification and dephosphorization
Inactive Publication Date: 2017-11-17
北京博达展望科技有限公司
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Problems solved by technology
[0005] In the activated sludge biological treatment process, the contradiction between substrate competition and different sludge ages in the process of phosphorus and nitrogen removal makes the existing activated sludge phosphorus and nitrogen removal technology still have great troubles, making nitrogen and phosphorus removal The effect can not meet the satisfactory requirements, even can not meet the emission standards
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Embodiment 1
[0035] The preparation method of degrading the composite type bacterial agent containing phosphorus comprises the steps:
[0036] (1) Sampling
[0037] From the stable anaerobic\anoxic ASBF reactor in the laboratory, the activated sludge rich in more than 70% of denitrifying phosphorus removal bacteria was used as experimental samples
[0038] (2) Preparation of medium
[0039] Beef extract peptone medium: Add 10g of peptone, 3g of beef extract, 5g of NaCl, 20g of agar, add 1L of distilled water, adjust the pH value to 7.2, and sterilize at 120°C for 20-30 minutes under the pressure of 0.1Mpa to prepare the culture medium. base;
[0040] Phosphorus-deficient medium for acetate synthesis: CH 3 COONa 2g, Na 2 HPO 4 2H 2 O 23mg, CaCl 2 2H 2 O 11 mg, NH 4 CL 152.8 mg, MgSO 4 ·7H 2 O 81.12 mg, K 2 SO 4 17.83mg, 7g of HEPES buffer solution; 2mL of trace elements, add distilled water to make 1L solution, adjust the pH value to 7.2, and sterilize at 120℃ for 20-30 minute...
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The invention discloses a preparation method of a compound bacterium agent for degrading phosphorus and belongs to the field of preparation of phosphorous reducing bacterium agents. According to the method, common activated sludge is used as a matrix for culture of activated sludge strains, active bio-enzymes mainly containing protease, lipase and cellulose are added in a culture medium manner during strain culture, so that active bio-enzymes combine and react with active microorganisms, a preservative chemical agent is added for separation and curing of the active strains when the activity of active microorganism strains and the active enzymes is cultured to the optimal state, and the activity is sealed up for safekeeping. The mechanism and activity of the active microorganisms and the active enzymes cannot be damaged during separation and curing, the activity can be recovered quickly when the strains of the active microorganisms and the active enzymes are dissolved and released in water again, and the strains can be quickly combined in other active microorganisms to grow and reproduce. The initial debugging time of biological treatment of activated sludge can be shortened and the nitrogen and phosphorus removal effect can be realized quickly.
Description
technical field [0001] The invention relates to the field of bacterial agent preparation, in particular to a preparation method for degrading phosphorus-containing composite bacterial agent. Background technique [0002] Activated sludge method is the main method of wastewater biological treatment with activated sludge as the main body. Activated sludge process and its derivative improvement process are the most widely used methods for treating municipal sewage. The activated sludge method is to continuously inject air into the wastewater, and after a certain period of time, sludge-like flocs are formed due to the reproduction of aerobic microorganisms. It can remove dissolved and colloidal biodegradable organic matter, suspended solids and other substances that can be adsorbed by activated sludge from sewage, and can also remove a part of phosphorus and nitrogen. [0003] In the process of biological treatment of activated sludge, organic nitrogen is converted into ammoni...
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Login to View More IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20
Inventor 陈勇
Owner 北京博达展望科技有限公司