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Method based on combination of gold nanoclusters and NGF siRNA for treating pancreatic cancer

A gold nano-cluster, nano-cluster technology, applied in DNA/RNA fragments, gene therapy, recombinant DNA technology, etc., to achieve the effect of improving stability, improving therapeutic effect, and simple preparation method

Active Publication Date: 2017-11-21
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free siRNA is easily degraded by nucleases in serum, therefore, it is also necessary to further develop the NGF siRNA-based pharmaceutical composition and preparation with better stability for preventing and / or treating cancer

Method used

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  • Method based on combination of gold nanoclusters and NGF siRNA for treating pancreatic cancer
  • Method based on combination of gold nanoclusters and NGF siRNA for treating pancreatic cancer
  • Method based on combination of gold nanoclusters and NGF siRNA for treating pancreatic cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0073] This example is used to illustrate the NGF siRNA combined with gold nanocluster (GNC-siRNA) of the present invention and its preparation method.

[0074] Prepare GNC-siRNA of the present invention through the following steps (technical route such as figure 1 shown):

[0075] (1) to HAuCl at 25°C 4 Solution added reducing agents GSH and CR 9 , where HAuCl 4 100mM, GSH 150mM, CR 9 75mM, equivalent to HAuCl 4 The molar ratio with GSH is 1:1.5, HAuCl 4 with CR 9 The molar ratio was 1:0.75, and then heated to 70° C. and stirred at constant temperature for 24 hours to obtain a gold nanocluster solution.

[0076] The color of the gold nanocluster solution is light yellow-green (such as figure 2 shown. Due to the publication of patents, figure 2 Incorrect colors may be displayed, this does not limit the invention). Using TEM to observe the size and surface morphology of gold nanoclusters, the TEM images are as follows image 3 As shown, its average diameter is 2.6...

Embodiment 2

[0079] Example 2 ～ 10

[0080]This example is used to illustrate the GNC-siRNA of the present invention and its preparation method.

[0081] Prepare the GNC-siRNA of embodiment 2~10 according to the method identical with embodiment 1, difference is only, the mass ratio of the NGF siRNA in step (2) and gold nanocluster is respectively 0.5:1,1:1,1.5: 1, 2:1, 2.5:1, 3.5:1, 4:1, 5:1, 100:1.

[0082] Use a zeta potential meter to measure the zeta potential of the above GNC-siRNA, see the results Figure 5 . Wherein, the gold nanocluster solution in step (2) is used as a mass ratio of 0:1. From Figure 5 It can be seen that when the NGF siRNA / GNC mass ratio is greater than 3, the zeta potential of the solution does not change, indicating that the siRNA reaches a saturated state. Therefore, the siRNA loading capacity of GNC-siRNA can reach up to 3000 mg siRNA / g gold nanoclusters, which is equivalent to 226 μmol siRNA / g gold nanoclusters.

[0083] It can be seen that, in th...

Embodiment 12

[0091] This example is used to illustrate the GNC-siRNA of the present invention and its preparation method.

[0092] Prepare GNC-siRNA of the present invention through the following steps (technical route such as figure 1 shown):

[0093] (1) to HAuCl at 26°C 4 Solution added reducing agents GSH and CR 9 , where HAuCl 4 100mM, GSH 300mM, CR 9 150mM, equivalent to HAuCl 4 The molar ratio with GSH is 1:3, HAuCl 4 with CR 9 The molar ratio is 1:1.5. Then heated to 70° C. and stirred at constant temperature for 36 hours to obtain a gold nanocluster solution. The color of the gold nanocluster solution is light yellow-green. Using TEM to observe the size and surface morphology of the gold nanoclusters, the average diameter is 2.89 nm, and the dispersion is good. The surface of the gold nanoclusters is positively charged as measured by a zeta potential meter, and the zeta potential is 20.1 mV.

[0094] (3) Add NGF siRNA to the gold nanocluster solution, and vortex and sti...

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Abstract

The invention provides a NGF siRNA-combined gold nanocluster. The NGF siRNA-combined gold nanocluster comprises a gold nanocluster body and NGF siRNA which are combined through the electrostatic adsorption effect. The invention further provides a preparation method of the NGF siRNA-combined gold nanocluster, a drug composition and a reagent box containing the NGF siRNA-combined gold nanocluster and application of the NGF siRNA-combined gold nanocluster in preparation of drugs used for preventing and / or treating cancer. According to the NGF siRNA-combined gold nanocluster, the siRNA stability can be improved, the body-interior circulation time is prolonged, the target ability of pancreatic tumor is improved, the mum effect of NGF is improved, and the anti-tumor treating effect of pancreatic cancer is improved; and the preparation method is simple, the reaction condition is mild, and a new pathway is developed for treating of the pancreatic cancer.

Description

technical field [0001] The invention relates to a gold nanocluster, in particular to an NGF siRNA combined gold nanocluster, a pharmaceutical composition and a kit containing the NGF siRNA combined gold nanocluster, and a preparation method and application of the NGF siRNA combined gold nanocluster. Background technique [0002] Gold nanoclusters (GNC) have significant Coulomb blocking effect and special magnetic properties, and the electronic structure similar to semiconductors also makes it have special fluorescent properties, so it is used in bioimaging and biomolecules (DNA, protein, enzyme) detection, etc. The reported applications of gold nanoclusters are, for example: gold nanoclusters modified with TAT polypeptide, which can be used in photodynamic therapy for fluorescence imaging, gene delivery and near-infrared light excitation; gold nanoclusters are loaded with polyacrylic acid and calcium phosphate to provide enhanced Fluorescent properties and can be used for c...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K9/51A61K31/713A61K47/02A61P35/00A61K33/242
CPCA61K9/5115A61K31/713A61K33/242A61K45/00A61K9/14C12N15/11C12N15/63
Inventor 蒋兴宇雷祎凤谢阳州昀
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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