Preparation method of mold spore product

A technology for mold spores and spores, which is applied in the field of preparation of mold spore products, can solve the problems of easy reduction of vitality, inconvenient storage and transportation, time-consuming and laborious elution of spores, etc.

Inactive Publication Date: 2017-11-21
武汉工控工业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the domestic standards for anti-mold experiments, most of them use traditional PDA medium for mold cultivation. The activation and cultivation process takes a long time and the sporulation rate is low.
At the same time, the preparation process of the spore suspension is cumbersome and complicated, the spore elution is time-consuming and laborious, and the efficiency is difficult to improve
[0004] In addition, the storage time for the spore suspension is required to be no more than 14 days, and the storage period is relatively short
The relevant spore suspension products currently on the market have problems such as short storage time, easy reduction of vitality, uncontrollable performance, and inconvenient storage and transportation, which have brought great inconvenience to actual production.

Method used

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  • Preparation method of mold spore product
  • Preparation method of mold spore product
  • Preparation method of mold spore product

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Embodiment Construction

[0023] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0024] 1. Selection of training methods

[0025] Inoculate ten strains of Aspergillus niger, Aspergillus terreus, Aspergillus flavus, Aspergillus versicolor, Paecilomyces variegae, Cladophyllum brevis, Penicillium fungus, Penicillium ochrevi, Trichoderma globosa, Trichoderma viride, etc. Potato agar plate, potato slant, potato agar eggplant bottle, bran flat plate, bran flat triangular flask culture, culture at 28°C. Observe the growth and growth speed, compare the sporulation yield when the spores are mature, and the difficulty of elution and separation, so as to choose the best culture method.

[0026] The growth rate of the mold is shown in Table 1.

[0027] The growth situation of each strain in table 1 in different media

[0028]

[0029] ...

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Abstract

The invention relates to a preparation method of a mold spore product. The preparation method comprises the following steps: inoculating molds onto a solid culture medium containing millet and bran; eluting mold spores from the solid culture medium by using an elution solution after the molds are grown fully on the surface of the solid culture medium, so as to obtain a spore elution solution; filtering and centrifugally washing the spore elution solution to obtain a spore precipitate; then processing the spore precipitate into the mold spore product. By using the bran and the millet as the culture medium, the sporulation quantity is greatly increased, and the maturation time of the spores is shortened. In addition, compared with a conventional spore suspension product, the spore powder product has the advantages that the storage time is greatly extended, and the spore suspension concentration is basically not changed after re-dissolution. Storage and transportation are carried out in a form of the spore powder product, so that great convenience is provided, and spattering and leakage of the spore suspension during storage and transportation can be effectively avoided.

Description

technical field [0001] The invention relates to the field of mold spore culture, more particularly, to a method for preparing a mold spore product. Background technique [0002] Mold is a kind of microorganism widely distributed in nature, and it is easy to grow in an environment with high humidity and high temperature. When mold grows in large quantities on the surface of military equipment, paint film, packaging, electronic equipment, etc., it will not only affect its appearance, but also cause related equipment failures and affect the normal operation of the equipment. Therefore, the various research work that prevents the growth of mold has been attached great importance to both at home and abroad. The mold test is an effective means to test the mold resistance of related products and materials. Countries around the world have also successively formulated relevant mold test standards, which all involve the cultivation of relevant molds and the preparation methods of mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00
CPCC12N3/00
Inventor 邓小华李震宇郑巧双罗茜余薇高奋刘婷潘笃杰付晓超
Owner 武汉工控工业技术研究院有限公司
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