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Fast culture method of ficus tikoua

A cultivation method and technology for loquat, which are applied in the field of rapid cultivation of loquat, can solve the problems of long rooting time, short period and high reproduction coefficient of rooting medium, and achieve high seedling quality, browning control and high survival rate. Effect

Inactive Publication Date: 2017-11-24
兰溪市顺光园艺技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopt this method to carry out ground loquat tissue culture, not only period is short, reproduction coefficient is high, cost is low, also has very strong factory production capacity, and utilize method of the present invention to propagate, can protect and develop and utilize ground loquat more effectively, but The rooting medium takes a long time to root, and there is no antibacterial agent in the medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The rapid culture method of ground loquat comprises aseptic germination of seeds, induction of callus, rooting culture, and the specific steps are:

[0019] 1) Bud pretreatment: Select the 2cm-long terminal buds or axillary buds with stems of the ground loquat, wash them with running water for 15 minutes, then disinfect them with 0.08% mercury solution for 10 minutes, 70% alcohol for 40 seconds, and then use Rinse with sterile water. In this step, the mercury solution and alcohol solution can eliminate the virus and bacterial substances on the ground loquat buds, control the ground loquat bud contamination, obtain sufficient sterile materials, and improve the survival rate of tissue culture. This is the ground loquat bud. The key to rapid cultivation of loquat;

[0020] 2) Induction of callus: Inoculate the treated ground loquat buds on MS medium containing 1 mg / L 6-BA and 0.4 mg / L NAA, and culture them at 23°C and 10 hours of light per day until Obtain the ground loqu...

Embodiment 2

[0024] The rapid cultivation method of ground loquat, concrete steps are:

[0025] 1) Select the 3cm-long terminal buds or axillary buds with stems of the ground loquat, wash them with running water for 13 minutes, then disinfect them with 0.1% mercury solution for 9 minutes, 75% alcohol for 35 seconds, and then rinse them with sterile water Clean, the mercury solution and alcohol solution in this step can eliminate the virus and bacterial substances existing on the loquat buds, control the contamination of the loquat buds, obtain sufficient sterile materials, and improve the survival rate of tissue culture, which is the basis for the rapid cultivation of loquat The essential;

[0026] 2) Inoculate the treated ground loquat buds on MS medium containing 1.5mg / L 6-BA and 0.35mg / L NAA, and cultivate them at a temperature of 25°C and under the condition of 9 hours of light per day until the height of 3cm is obtained. The ground loquat seedlings can be obtained. In this step, the ...

Embodiment 3

[0030] The rapid cultivation method of ground loquat is as follows: select the 3cm-long terminal bud or axillary bud with stem section of ground loquat, wash it with running water for 15 minutes, then disinfect it with 0.12% mercury solution for 7 minutes and 70% alcohol for 32 seconds. Then rinse it with sterile water, and inoculate the treated ground loquat buds on MS medium containing 1.8mg / L 6-BA and 0.4mg / L NAA, under the conditions of 27°C and 8h of light per day Cultivate until the ground loquat seedlings with a height of 4cm are obtained, cut the seedlings, and transfer them to the ingredients and their parts by weight: 0.003 parts of IBA, 0.0003 parts of 6-BA, 0.0002 parts of bacteriostatic agent, 1.1 parts of potassium nitrate, and 1.2 parts of ammonium nitrate , 0.19 parts of magnesium sulfate, 0.08 parts of dipotassium hydrogen phosphate, 0.27 parts of calcium chloride, 0.17 parts of trace elements, 0.0018 parts of vitamin C, 0.001 parts of vitamin B, 0.003 parts of...

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Abstract

The invention discloses a fast culture method of ficus tikoua. The fast culture method comprises the following specific steps: selecting terminal buds or axillary buds with stems of ficus tikoua, washing the terminal buds or the axillary buds to be clean with sterile water after sterilization, inoculating the terminal buds or the axillary buds on an MS culture medium containing 6-BA (benayl aminopurine) and NAA (N-acetylaspartate) for culture, so as to obtain young seedlings of ficus tikoua; cutting off the young seedlings and inoculating the young seedlings into rooting culture solution for culture, so as to obtain seedlings of ficus tikoua with 3-5 main roots which are 3-5 cm long; exercising the seedlings and transplanting the seedlings. The fast culture method has the beneficial effects that the germination rate and the rooting rate of ficus tikoua are greatly improved, the defect of the traditional method that propagation is slow is overcome, ficus tikoua can be propagated fast, and the propagation cycle of ficus tikoua is shortened, so that propagated progenies are neat and consistent, and the excellency of a variety is maintained; the young seedless of ficus tikoua, which are obtained by the culture method, is high in survival rate of cultivation, high in quality of the seedlings and high in disease resistance; and the culture method is not influenced by any natural factor, and therefore, ficus tikoua can be planted throughout the year on a large scale.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a rapid culture method of loquat. Background technique [0002] Ground loquat (Ficus tikoua Bur), also known as ground fruit, sweet potato, and ground pomegranate, is a creeping woody vine of the genus Ficus in the family Moraceae. There are two varieties of ground loquat, one kind of fruit is sweet and delicious, and can be eaten; the other has no fragrance and cannot be eaten , generally a piece of the same species. Loquat is mainly produced in Guangxi, Guizhou, Hunan and other places, and is used as a traditional medicinal material of the Miao people in Guizhou and Xiangxi. Pharmacological studies on this genus at home and abroad show that this genus mainly contains chemical components such as terpenoids, flavonoids, coumarins, alkaloids, etc. , smooth muscle relaxation, anti-tumor, antibacterial and other effects, this genus of plants has potential development value ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨洋
Owner 兰溪市顺光园艺技术有限公司
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