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Anaphalis bulleyana cultivation and breeding method

A technology of vanilla and its operation method, which is applied in the field of cultivation and reproduction of medicines, can solve the problems that wild vanilla is on the verge of extinction and cannot be found, and achieve the effects of slow reproduction speed, stable cultivation and reproduction, and high economic benefits

Inactive Publication Date: 2017-02-15
云南金碧制药有限公司
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Problems solved by technology

[0003] Noon vanilla is mainly distributed in Santai, Tiesuo, Sanchahe, Shiyang's high mountain shady ditches, Qingbian, grass slopes and low mountain grasslands in Dayao County. It is a habitual and commonly used medicine of the Yi people. However, due to the successive years of drought in recent years, the wild Herb No. Propagation and production of Tissue Culture

Method used

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Embodiment 1

[0029] 1. Method for controlling the contamination of the explants of Herba Sinensis L.

[0030] The operating procedures for pollution control are: explants—leaves—remove debris with a brush—soak in saturated detergent liquid for 5mim—rinse with clean tap water for 30 minutes—rinse with distilled water for 5 times—move into an ultra-clean workbench —— Use 75% alcohol for disinfection for 10-15s → rinse with sterile water for 5 times → 0.1% HgCl2 (add Tween-80 dropwise, 20 drops per liter) for 3-5 minutes → wash with sterile water for 8-10 times, The rate of contamination of the explants can be controlled.

[0031] 2. The technical system of the disinfection method for the explants of Herba sativa

[0032] The operation method of the disinfection treatment of the explants of the No. 1 herb: In the afternoon on a sunny day, after cutting the young leaves of No. 1, use brushes, toothbrushes, surgical scissors and other tools to gently remove the sundries on the surface of the y...

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Abstract

The invention relates to a plant cultivation and breeding method, in particular to a drug cultivation and breeding method. The method includes the operation steps of: a. Anaphalis bulleyana explant disinfection treatment; b. operation of a sterile ultra-clean workbench; c. cultivation of parenchyma cells; d. propagation culture of Anaphalis bulleyana explant callus; e. culture of Anaphalis bulleyana stem-leaf differentiation seedlings; f. Anaphalis bulleyana rooting culture; and g. seedling hardening and transplanting. Through repeated tests, the seedling culture success rate and the survival rate after transplanting both reach 90% or more, therefore the method achieves stable cultivation and breeding of Anaphalis bulleyana, reaches the purposes of low investment and high economic benefits, and solves the extinction risk of Anaphalis bulleyana due to low seed yield and slow breeding speed.

Description

technical field [0001] The invention relates to a method for cultivating and propagating plants, in particular to a method for cultivating and propagating medicines. Background technique [0002] Anaphalis bulleyana(J.F.Jaffr.)Chang[Pluchea bulleayanaJ.F.Jeffr.], also known as: Cyperus cyperus, wild spicy tobacco, five-spice grass, and the Yi people call it "poetry of nest octopus", which is a plant of the genus Asteraceae The whole herb of velvet hair, pungent and warm, enters the stomach and lung meridians, has the effects of clearing away heat and dampness, relieving cough, cutting off malaria, reducing inflammation and pain, invigorating the stomach and promoting qi. It is mainly used for pharyngitis, wind-heat cold, tonsillitis and bronchitis , acute gastroenteritis, urinary tract infection, cystitis, urethritis, tracheitis, pertussis, infantile malaria, malaria and other common diseases, not only widely used in traditional Chinese medicine decoction, but also a lot of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 陈蕾
Owner 云南金碧制药有限公司
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