Process for producing casein by hydrochloric acid and citric acid mixed acid method
A technology of hydrochloric acid, citric acid, and mixed acids, which is applied in the field of casein, can solve the problems of no sterilization measures and affect the microbial indicators of casein products, and achieve the effects of short production cycle, improved product yield and quality, and simple preparation process
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Embodiment 1
[0024] In the embodiment of the present invention, the process for producing casein by hydrochloric acid citric acid mixed acid method comprises the following steps:
[0025] 1) Weigh the rice bran, mix and heat it with 0.5% sodium hydroxide solution with a mass concentration three times its mass, heat to 45° C. and keep stirring for 2 hours to fully dissolve the protein, and prepare mixture A.
[0026] 2) Pass the mixture A through double-layer gauze to remove impurities in the feed liquid to obtain a filtrate.
[0027] 3) Add the mixed acid solution to the filtrate while stirring until the pH of the solution is 4, add the mixed acid solution in 10 minutes, and continue to stir for 10 minutes after adding, to prepare the mixture B; wherein the mixed acid solution contains 0.3% hydrochloric acid and Citric acid with a mass concentration of 0.1%.
[0028] 4) Centrifuge the mixture B, take the precipitate, wash the precipitate with water, and centrifuge twice to obtain the prec...
Embodiment 2
[0031] In the embodiment of the present invention, the process for producing casein by hydrochloric acid citric acid mixed acid method comprises the following steps:
[0032] 1) Weigh the rice bran, mix and heat it with 1.5% sodium hydroxide solution with a mass concentration 10 times its mass, heat to 55° C. and keep stirring for 5 hours to fully dissolve the protein, and prepare mixture A.
[0033] 2) Pass the mixture A through double-layer gauze to remove impurities in the feed liquid to obtain a filtrate.
[0034] 3) Add the mixed acid solution while stirring in the filtrate until the solution pH5, add the mixed acid solution in the time of 20min, and continue to stir for 50min after adding, to prepare the mixture B; wherein the mixed acid solution contains 0.8% hydrochloric acid and Citric acid with a mass concentration of 0.8%.
[0035] 4) Centrifuge the mixture B, take the precipitate, wash the precipitate with water, and centrifuge for 5 times to obtain the precipitat...
Embodiment 3
[0038] In the embodiment of the present invention, the process for producing casein by hydrochloric acid citric acid mixed acid method comprises the following steps:
[0039] 1) Weigh the rice bran, mix and heat it with 0.8% sodium hydroxide solution with a mass concentration 5 times its mass, heat to 48° C. and keep stirring for 3 hours to fully dissolve the protein, and prepare mixture A.
[0040] 2) Pass the mixture A through double-layer gauze to remove impurities in the feed liquid to obtain a filtrate.
[0041] 3) Add the mixed acid solution to the filtrate while stirring until the pH of the solution is 4.2, add the mixed acid solution in 12 minutes, and continue to stir for 20 minutes after the addition, to prepare mixture B; wherein the mixed acid solution contains 0.4% of the mass concentration Hydrochloric acid and citric acid with a mass concentration of 0.2%.
[0042] 4) Centrifuge the mixture B, take the precipitate, wash the precipitate with water, and centrifug...
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