Preparation method for maidenhair total flavone extract and application of maidenhair total flavone extract in antidepressant drug
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A malachite grass and extract technology, which can be used in drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve problems such as no anti-depression yet.
Active Publication Date: 2017-11-24
蔡德成 +1
View PDF4 Cites 6 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] Due to the pressure of work and social life, the incidence of depression is relatively high in our countr...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0015] Embodiment 1 preparation of total flavonoids of Malachite
[0016] Grind 1 kg of peacock grass (whole plant), pass through a 10-mesh sieve, use water as a solvent, extract twice, each time the amount of solvent is 8 times the mass of the medicinal material (weight ratio), and warm soak at 70°C for 1 hour each time. Combine the extracts, concentrate to about 1.5 times the mass of the medicinal materials (about 1.5 liters), add 95% ethanol (ethanol / water volume ratio) to adjust the ethanol concentration to 70% (ethanol / water volume ratio), and store it in refrigeration at 4°C After 12 hours, centrifuge and take the supernatant. The obtained supernatant was extracted 3 times with water-saturated n-butanol, each time the volume of the concentrated solution was 1 / 2, the n-butanol extract was combined, concentrated into an extract, and dried under reduced pressure to obtain the total flavonoids extract of Malachite 110.7g, and the total flavonoids measured are 50.2g, a...
experiment example 2
[0017] Experimental Example 2 Study on the Antidepressant Effect of Total Flavonoids of Malachite
[0018] 1. Experimental materials and methods
[0019] 1.1 Materials and Instruments
[0020] Healthy male KM mice, weighing 18g-22g, were provided by the Animal Experiment Center of Dalian Medical University, certificate number: 2008-0002, and the total flavonoids of Malacock grass used were self-made by the above-mentioned process. Desipramine (DMI, lot number: 123K1364, positive drug), apomorphine (Apomorphine, APO, lot number 110K1391) and yohimbine (Yohinbin, lot number: 124F0516) are all products of Sigma, USA. ); YLS-1A multifunctional mouse autonomic activity recorder (Shenyang Baiyi Technology Development Co., Ltd.).
[0021] 1.2 Animal grouping
[0022] 40 KM male mice were randomly divided into 4 groups: blank group, positive drug group (desipramine) and low (150mg / kg) and high-dose (300mg / kg) groups of malachite total flavonoids (ZHT). Group 10. ZHT and posit...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
Granularity
aaaaa
aaaaa
Weight
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses application of a maidenhair total flavone extract in preparation of an antidepressant drug. A preparation method of the maidenhair total flavone extract comprises the steps that (1) through an aqueous solution, maidenhair is extracted at least one time to obtain an extracting solution, and the extracting solution is concentrated to obtain a concentrated solution; (2) 95% alcohol (alcohol/water volume ratio) is added to the concentrated solution for adjustment so as to enable the alcohol concentration to be 70% (alcohol/water volume ratio), then the concentrated solution is refrigerated at the temperature of 4 DEG C and stored for 12-24 h, centrifugation is conducted, and a supernatant is taken; (3) the supernatant is extracted through water-saturated normal butanol, a normal butanol extracting solution is merged, concentrating and drying are conducted, and then the maidenhair total flavone extract is obtained. According to the maidenhair total flavone extract, the weight content of total flavone measured according to the colorimetric method is higher than 40%. Through a pharmacological test, it is certified that the maidenhair total flavone extract has a high antidepressant effect and is high in purity, simple, convenient and practical in preparation method, suitable for industrial production and capable of serving as a crude drug separately or by combining other drugs to be applied to preparation of the antidepressant drug.
Description
technical field [0001] The invention relates to a herbal medicine and application thereof, in particular to an extract with total flavonoids of malachite as active ingredients and its application in the preparation of antidepressant medicines. Background technique [0002] Peacock grass (Tagetes patula L.), also known as red yellow grass, rattan chrysanthemum, and small marigold, is a plant of the genus Marigold in the Asteraceae family. It is an annual herb with a flowering period from July to September. It is cultivated all over our country and is a common ornamental flower. Peacock grass has medicinal and health care functions. The flowers and leaves can be used as medicine. Can treat whooping cough, tracheitis, cold and so on. [0003] Since the 1980s, people began to study the chemical constituents of Malachite, and isolated flavonoids such as patuletin, quercetagetin, and quercetagitrin ( State Administration of Traditional Chinese Medicine "Chinese Materia Medica" ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more