A method for jointly extracting igy, phosvitin, lecithin, egg oil and defatted egg yolk powder from egg yolk

A high yolk protein and combined extraction technology, applied in the field of egg deep processing, can solve the problems of complex composition of egg yolk, difficulty in making the best use of everything, and few researches on comprehensive utilization of egg yolk, etc. The effect of high oil lift

Active Publication Date: 2020-08-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the complex composition of egg yolk, it is difficult to make the best use of it
At present, research mainly focuses on the separation and extraction of single bioactive components in egg yolk, and there is little research on the comprehensive utilization of egg yolk

Method used

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  • A method for jointly extracting igy, phosvitin, lecithin, egg oil and defatted egg yolk powder from egg yolk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 500ml egg yolk stock solution, add 9 times volume of distilled water to dilute, adjust pH=5.5, stir well and let stand overnight in a refrigerator at 4℃, take it out and centrifuge at 10000rpm for 15min to obtain supernatant A and egg yolk slurry; Add ammonium sulfate and sodium chloride to supernatant A to precipitate IgY, so that the final weight concentrations of the two reach 28% and 0.5%, respectively. Then, the precipitate obtained by centrifugation is completely dissolved in distilled water, and the crude IgY aqueous solution is obtained after dialysis to remove salt. Then add PEG6000 to the crude IgY aqueous solution and adjust the pH=5.0. The final weight concentration of PEG6000 is 8%, put it in the refrigerator for 1h and centrifuge to obtain the purified IgY precipitate. Finally, the purified IgY precipitate is re-dissolved in distilled water and dialyzed Remove PEG6000 to obtain purified IgY aqueous solution, which can be lyophilized to obtain IgY fi...

Embodiment 2

[0029] (1) Take 500ml of egg yolk stock solution, add 3 times volume of distilled water to dilute, adjust pH=4, stir well and let stand overnight in a refrigerator at 4℃, rotate at 10000rpm, centrifuge for 15min, obtain supernatant A and egg yolk slurry; then supernatant Solution A was added with ammonium sulfate and sodium chloride to precipitate IgY, so that the final concentration reached 20% and 1%, respectively, and then the precipitate obtained by centrifugation was completely dissolved in distilled water, and the crude IgY aqueous solution was obtained after dialysis and desalination, and then added to the crude IgY aqueous solution Add PEG6000 and adjust the pH=7.0, the final concentration of PEG6000 is 4%, put it in the refrigerator for 1h and centrifuge to obtain the purified IgY precipitate. Finally, the purified IgY precipitate is dissolved in distilled water again, and the PEG6000 is removed by dialysis to obtain purified IgY. Aqueous solution, IgY finished product ...

Embodiment 3

[0036] (1) Take 500ml of egg yolk stock solution, add 12 times the volume of distilled water to dilute, adjust pH=7.0, stir well and let stand overnight at 4℃, rotate speed 10000rpm, centrifuge for 15min to obtain supernatant A and egg yolk slurry; then add supernatant A IgY was precipitated with ammonium sulfate and sodium chloride to make the concentration reach 35% and 0.1% respectively, and then the precipitate obtained by centrifugation was completely dissolved in distilled water, and the crude IgY aqueous solution was obtained after dialysis to remove the salt, and then PEG6000 was added to the crude IgY aqueous solution and Adjust pH=3.0, PEG concentration is 10%, put in the refrigerator for 1h and centrifuge to obtain purified IgY precipitate. Finally, the purified IgY precipitate is dissolved in distilled water again, and dialyzed out PEG6000 to obtain purified IgY aqueous solution, which is freeze-dried IgY finished products can be obtained;

[0037] (2) Add 400ml of ab...

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Abstract

The invention discloses a method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk. The method comprises the following steps: firstly, diluting yolk fluid by using water and centrifuging; salting out the acquired supernatant and performing polyethylene glycol sediment, thereby extracting IgY; for the yolk pulp on the bottom layer, removing water from the yolk pulp by adopting ethyl alcohol, extracting total yolk lipid by utilizing an ethyl alcohol and ethyl acetate mixed solvent and treating by utilizing ethyl acetate, thereby acquiring two products of lecithin and egg oil; treating the egg white by utilizing a sodium chloride solution after removing the lipid, thereby acquiring the phosvitin. According to the method, the technological operation is simple and convenient, environment-friendly and non-toxic effects are achieved, the production cost is low, the product purity and yield are higher and a technical support is supplied for the comprehensive utilization of yolk.

Description

Technical field [0001] The invention belongs to the field of deep processing of egg products, in particular to a method for jointly extracting IgY, egg yolk high phosphate protein, lecithin, egg butter and defatted egg yolk powder from egg yolk. Background technique [0002] Egg yolk is the most nutritious part of the egg. The protein content is not only high, and its amino acid composition is very similar to human protein. It is a high-quality full-price protein second only to cow's milk, and its biological potency is as high as 96%; secondly, enrichment The lipids in egg yolks not only contain rich unsaturated fatty acids and suitable linoleic acid content, but also contain phospholipids that are indispensable to humans in the brain and nervous system. Therefore, the proteins and lipids in egg yolks The development of active ingredients has always been a research focus of domestic and foreign scholars. [0003] Due to the complex composition of egg yolk, it is difficult to make ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/02C07K14/465C07F9/10C11B1/10
CPCC07F9/103C07K14/465C07K16/02C11B1/10C11B1/108
Inventor 蔡朝霞罗进旭刘巧唐世涛付星马美湖
Owner HUAZHONG AGRI UNIV
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