Gene marker and detection method for detecting benign and malignant liver tumors, and kit

A gene marker, liver tumor technology, applied in the direction of biochemical equipment and methods, microbial determination/examination, etc., can solve the problems of patients' mental burden, patient suffering, diagnosis difficulties, etc., and achieve easy dynamic monitoring and good user experience. , high sensitivity and specificity

Pending Publication Date: 2017-11-24
SHANGHAI EPICAN BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the contrary, if a benign tumor is treated as a malignant tumor, it will not only cause the patient's mental burden, but also cause the patient to suffer and suffer losses due to the adoption of many unnecessary treatments.
However, some benign tumors and malignant tumors have similar growth forms, which cause great difficulties in diagnosis.

Method used

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  • Gene marker and detection method for detecting benign and malignant liver tumors, and kit
  • Gene marker and detection method for detecting benign and malignant liver tumors, and kit
  • Gene marker and detection method for detecting benign and malignant liver tumors, and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Screening of Liver Tumor Gene Markers

[0026] 1) Extract plasma DNA:

[0027] 10 ng of plasma DNA was extracted from samples from 20 patients with liver cancer and 20 patients with benign liver tumors. This step can be carried out using any methods and reagents suitable for extracting plasma DNA well known to those skilled in the art.

[0028] 2) End-fill the plasma DNA, overhang A and connect with the sequencing adapter:

[0029] Prepare a reaction mixture containing 50uL plasma DNA, 7uL End Repair & A-Tailing Buffer and 3uL End Repair & A-Tailing Enzyme mix according to the Kapa Hyper Perp Kit instructions (the total volume is 60uL), incubate at 20°C for 30 minutes, and then incubate Incubate at 65°C for 30 minutes. Prepare the following ligation reaction mixture in a 1.5mL low adsorption EP tube: 5uL Nuclease free water, 30uL Ligation Buffer and 10 uL DNA Ligase. Add 5uL of sequencing adapters to 45uL ligation reaction mixture, mix, heat at 20°C for 2...

Embodiment 2

[0048] Example 2. Effectiveness of Liver Tumor Gene Markers

[0049] This example verifies the effectiveness of the liver tumor gene markers of the present invention for distinguishing benign from malignant liver tumors.

[0050] According to the method of Example 1, the 5-hmC content of the 10 liver cancer gene markers described in the present invention in the first batch of 164 samples (82 cases of liver cancer and 82 cases of benign liver tumors) was determined.

[0051]The standardized 5-hmC content of each gene marker is multiplied by the corresponding weighting coefficient of the marker in Example 1 to obtain the predictor t of the gene marker, and then the predictor t of each gene marker is added to obtain The total predictor T, and then the total predictor T is transformed according to the following formula to obtain the score P:

[0052]

[0053] If P>0.5, the subject sample suffers from liver cancer; if P≤0.5, the subject suffers from benign liver tumor.

[0054...

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PUM

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Abstract

The invention relates to a gene marker and a detection method for detecting benign and malignant liver tumors, and a kit. The gene marker comprises one or two or more of the following genes: FAT1, ESRRG, SOX9, EVC, AXDND1, RELN, TF, TNFRSF11B, PDX1, and ORM2. The content of 5-hydroxymethylcytosine in the liver tumor gene marker is detected through high-throughput sequencing, so as to determine a benign or malignant liver tumor. The gene marker for detecting benign and malignant liver tumors has the advantages of being safe and noninvasive, having a wide DNA source, being highly accurate, being convenient to operate, and providing good user experience. The gene marker can be combined with other clinical indexes, and provides more accurate determining results for following screening, diagnosis, treatment and prognosis of liver tumors.

Description

technical field [0001] The invention relates to the technical field of clinical molecular diagnosis of benign and malignant liver tumors. Specifically, the present invention relates to gene markers, kits and methods for detecting benign and malignant liver tumors. Background technique [0002] Tumors are divided into benign tumors and malignant tumors. Benign tumors grow slowly and are generally harmless to health and life, except for occupying vital parts. Malignant tumors grow rapidly, compete with the human body for nutrients, produce harmful metabolites, destroy the normal organ tissue structure of the human body, and are extremely harmful to human health. If not treated in time, it will kill people. Common benign liver tumors include hepatocellular adenoma, hepatic duct cell adenoma, adrenal residual tumor, hemangioma, and hamartoma. Liver cancer refers to hepatocellular carcinoma, which is what we usually call liver cancer. Liver cancer is one of the most common ma...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/112C12Q2600/154
Inventor 陆星宇宋艳群彭莱张子谋
Owner SHANGHAI EPICAN BIOTECH CO LTD
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