Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly analyzing lipid components in bee pollen

A technology for rapid analysis of bee pollen, applied in the field of chemical analysis, to achieve the effect of eliminating interference, high resolution and sensitivity

Active Publication Date: 2017-11-24
杭州厚爱生物科技有限公司
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology has high resolution and accurate qualitative, which overcomes the problems of low accuracy and reliability in component identification of previous technologies, and is a new and efficient technology for lipidomics analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly analyzing lipid components in bee pollen
  • Method for rapidly analyzing lipid components in bee pollen
  • Method for rapidly analyzing lipid components in bee pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The UPLC-Q-Exactive Orbitrap MS detection method of lipid component in camellia bee pollen, its method is as follows:

[0036] (1) Sample pretreatment:

[0037] ① Weigh 5-10g camellia bee pollen, grind and pulverize it, add a certain amount of pollen lipid extract, vortex, let stand, and then centrifuge, repeat three times, centrifuge for the last time, after the solution is layered, take the lower organic phase and place in new tubes;

[0038] ② Add deionized water, conduct secondary extraction on the pollen organic layer, vortex, let it stand, repeat three times, after sufficient extraction, centrifuge, take out the lower organic phase into a new tube, dry the organic solvent under the protection of nitrogen until it is transparent Thin film, dried and re-dissolved in pollen lipid extract, to be tested;

[0039] (2) Utilize UPLC-Q-Exactive Orbitrap MS technology to carry out qualitative and quantitative analysis:

[0040] With chloroform:methanol (2:1, V / V) the sam...

Embodiment 2

[0048] The UPLC-Q-Exactive Orbitrap MS detection method of lipid component in lotus bee pollen, its method is as follows:

[0049] (1) Sample pretreatment:

[0050] ① Weigh 5-10g of lotus bee pollen, grind and crush it, add a certain amount of pollen lipid extract, vortex, let stand, and then centrifuge, repeat three times, centrifuge for the last time, after the solution is layered, take the lower organic phase and place in new tubes;

[0051] ② Add deionized water, conduct secondary extraction on the pollen organic layer, vortex, let it stand, repeat three times, after sufficient extraction, centrifuge, take out the lower organic phase into a new tube, dry the organic solvent under the protection of nitrogen until it is transparent Thin film, dried and re-dissolved in pollen lipid extract, to be tested;

[0052] (2) Utilize UPLC-Q-Exactive Orbitrap MS technology to carry out qualitative and quantitative analysis:

[0053] With chloroform:methanol (2:1, V / V) the sample is ...

Embodiment 3

[0061] The UPLC-Q-Exactive Orbitrap MS detection method of lipid component in rape bee pollen, its method is as follows:

[0062] (1) Sample pretreatment:

[0063] ① Weigh 5-10g of rapeseed bee pollen, grind and pulverize it, add a certain amount of pollen lipid extract, vortex, stand still and then centrifuge, repeat three times, centrifuge and stand for the last time, after the solution is layered, take the lower organic phase and place it in new tubes;

[0064] ② Add deionized water, conduct secondary extraction on the pollen organic layer, vortex, let it stand, repeat three times, after sufficient extraction, centrifuge, take out the lower organic phase into a new tube, dry the organic solvent under the protection of nitrogen until it is transparent Thin film, dried and re-dissolved in pollen lipid extract, to be tested;

[0065] (2) Utilize UPLC-Q-Exactive Orbitrap MS technology to carry out qualitative and quantitative analysis:

[0066] With chloroform:methanol (2:1,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
collision energyaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for rapidly analyzing lipid components in bee pollen. The method comprises the following steps: using an ultra performance liquid chromatography and quadrupole-static electric field orbitrap high resolution mass spectrum coupling technique (UPLC-Q-Exactive Orbitrap MS), and using positive and negative ion monitoring mode, full-scanning and the function of automatically triggering a tandem mass spectrometry scanning to accurately realize precise quantification of lipid components in bee pollen from different plants, and providing the basis for the origin place of bee pollen, authenticity judgment, doping counterfeiting and functional nutriology research of bee pollen. The technology is high in resolution ratio and accurate in positioning, overcomes the problems of low accuracy and reliability in ingredient identification in the prior art, and is a novel efficient technology for lipid component analysis.

Description

technical field [0001] The invention belongs to the field of chemical analysis, in particular to a method for rapidly analyzing lipid components in bee pollen. Background technique [0002] Lipids, also known as lipids, are insoluble in water but easily soluble in fat solvents (alcohols, ethers, chloroform, benzene) and other non-polar organic solvents, esters formed by the dehydration condensation of fatty acids and alcohols and their derivatives Substances are collectively referred to as lipids, which include fats, waxes, steroids, fat-soluble vitamins (such as vitamins A, D, E, and K), monoglycerides, diglycerides, phospholipids, etc. Its main physiological functions include energy storage, formation of cell membranes, and information transmission of membranes. Lipids are used today in the cosmetic and food industries. Lipids can be roughly divided into eight categories according to their amphipathic properties (both hydrophilic and hydrophobic): fatty acids, glycerides...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 王凯李强强吴黎明薛晓锋
Owner 杭州厚爱生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com