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Method for field planting of peach tree embryo culture seedlings in same year

A technology for cultivating embryo seedlings and peach trees, which is applied in the field of field planting of peach tree embryo seedlings in the same year, can solve the problems of restricting the breeding efficiency of early-maturing peaches, the low seedling rate of embryo cultivation, and the low survival rate of transplanting, so as to prevent the seedling Blight, increase the survival rate, the effect of strong plants

Active Publication Date: 2017-12-01
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, peach breeders have done a lot of work on the cultivation of early-maturing peach embryos, and have bred many excellent early-maturing varieties, but the seedling rate of embryo cultivation is low, and the imperfect transplanting technology of embryo-cultivated seedlings has seriously restricted the development of early-maturing peaches. Breeding efficiency
[0003] Under normal circumstances, peach seedlings rescued from embryos will enter secondary dormancy about 2 months after transplanting, the growth point will no longer grow, and the tree body will be short. Slow, resulting in thin seedlings when transplanted to the field, and low transplant survival rate

Method used

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  • Method for field planting of peach tree embryo culture seedlings in same year
  • Method for field planting of peach tree embryo culture seedlings in same year
  • Method for field planting of peach tree embryo culture seedlings in same year

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] The cultivation of embodiment 1 peach tree embryo culture seedling

[0090] In spring, the peach blossoms in the big bud stage are emasculated, and then the male parent's pollen is applied to the stigma of the female parent's flower, and the fruit is picked in the green ripening period (one week before maturity). In the ultra-clean work, 70% alcohol by volume fraction and 20% sodium hypochlorite by mass fraction were used to disinfect the fruit for 10 minutes, and inoculated into the test tube containing WPM medium to obtain inoculated embryos.

[0091] The inoculated embryos are placed in a dark environment at -4°C for 10 weeks, and then cultivated under natural light at 15-23°C until they germinate, and peach tree embryo seedlings are obtained.

Embodiment 2

[0093] Prepare the first mixed matrix according to the mass ratio of peat, perlite and vermiculite at 1:1:1, and autoclave at 121° C. for 30 minutes to obtain the product. The first mixed substrate is packed into a cultivation pot whose bottom air hole area accounts for 25% of the total bottom area.

[0094] Preparation of seedling culture medium: prepare the second mixed matrix according to the mass ratio of peat: perlite: vermiculite of 1.5:0.5:1, and sterilize under high pressure at 121°C for 30 minutes to obtain it; according to the volume and mass ratio of the second mixed matrix and slow-release fertilizer 1m 3 : 5kg mixed to get seedling culture medium.

[0095] The slow-release fertilizer is: Aibeishi Controlled-release fertilizer (garden fertilizer), according to mass percentage, including 14.00% of total nitrogen, phosphoric acid (P 2 o 5 ) 14.00%, potassium chloride 14.00%, magnesium 0.50%, sulfur 4.25%, copper 0.05%, iron 1.25%, manganese 0.05%, zinc 0.50%, mo...

Embodiment 3

[0103] Prepare the first mixed matrix according to the mass ratio of coconut peat, perlite and vermiculite at 0.8:0.6:0.9:1, and sterilize under high pressure at 121° C. The first mixed substrate is packed into a cultivation pot whose bottom air hole area accounts for 20% of the total bottom area.

[0104] Preparation of seedling culture medium: prepare the second mixed substrate according to the mass ratio of coconut peat: perlite: vermiculite of 1.2:0.5:1, and sterilize it by high-temperature steam; 3 : 4kg mixed to get seedling culture medium.

[0105] Described slow-release fertilizer is: according to mass percentage, comprises nitrogen total amount 12.00%, phosphoric acid (P 2 o 5 ) 12.00%, potassium chloride 12.00%, magnesium 0.20%, sulfur 4.00%, copper 0.02%, iron 1%, manganese 0.02%, zinc 0.30%, molybdenum 0.003%; wherein the mass of urea nitrogen accounts for 13% of the total nitrogen mass.

[0106] Get 12 days of germination among the embodiment 1, expand 2~3 true...

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Abstract

The invention provides a method for field planting of peach tree embryo culture seedlings in the same year. The method includes: transplanting the peach tree embryo culture seedlings into a mixed matrix; controlling water content in the matrix to 29-40%; using high-nitrogen water-soluble fertilizer for irrigation after seedling recovering; when the peach tree embryo culture seedlings are dormant, performing foliage application of gibberellins of 100-250mg / L to break dormancy; when the peach tree embryo culture seedlings grow to 20-30cm in height, transplanting the same into a mixed matrix containing slow-release fertilizer; when the peach tree embryo culture seedlings grow to 70-100cm in height, planting the same into a field. By controlling the water content in the matrix after transplanting, applying the high-nitrogen water-soluble fertilizer after seedling recovering, applying the slow-release fertilizer for seedling strengthening after transplanting for the second time and combining foliage application of gibberellins of 100-250mg / L after dormancy to break dormancy, the peach tree embryo culture seedlings are enabled to be robust in growth and developed in root system and can be planted in the field in the same year of cultivation, and survival rate of the peach tree embryo culture seedlings when being planted in the field is increased.

Description

technical field [0001] The invention relates to the technical field of peach tree seedling cultivation, in particular to a method for cultivating peach tree embryo seedlings for field planting in the same year. Background technique [0002] Embryo rescue technology is the early in vitro cultivation of embryos that are difficult to sow and become seedlings due to nutritional and / or physiological factors, or aborted or degenerated embryos appear during development. For early-maturing varieties of stone fruit trees, the embryos are usually underdeveloped. Sowing can not emerge or the germination rate is very low. At present, peach breeders have done a lot of work on the cultivation of early-maturing peach embryos, and have bred many excellent early-maturing varieties, but the seedling rate of embryo cultivation is low, and the imperfect transplanting technology of embryo-cultivated seedlings has seriously restricted the development of early-maturing peaches. Breeding efficienc...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00A01G17/00A01G7/06A01C21/00
Inventor 杜纪红叶正文苏明申周慧娟李雄伟张夏南
Owner SHANGHAI ACAD OF AGRI SCI
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