Freeze-dried powder prepared by enriching platelets and extracting active factors from placenta

Abstract

The invention relates to freeze-dried powder containing active factors. The active factors are obtained by enriching blood platelets from a placenta and conducting extraction. The active factors are selected from EGF, PDGF-BB, FGF, HGF and a combination thereof. The freeze-dried powder contains the active factors and a freeze-drying protective agent. The freeze-drying protective agent is selected from trehalose, mannitol, chitosan or a combination thereof. A method for enriching blood platelets from the placenta and extracting active factors comprises the steps of blood platelet enriching, blood platelet lysate preparation and freeze-drying and storage of blood platelet-derived active factors. The freeze-dried powder and the preparing method thereof have the beneficial effects as described in the description.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for enriching platelets and extracting active factors from placenta, in particular to a method for enriching platelets and extracting active factors from autologous placenta. The active factor prepared by the invention can be directly applied to the damaged skin surface or injected with microneedles for wound healing. More particularly, the present invention relates to a freeze-dried powder prepared by enriching platelets from placenta and extracting active factors such as but not limited to EGF, PDGF-BB, FGF, HGF and combinations thereof. Background technique [0002] In recent years, with the deepening of research on mesenchymal stem cells, its secreted factors have become a hot spot for researchers. Mesenchymal stem cells can secrete a variety of biologically active cytokines, which can effectively regulate body cell signal transduction, activate human stem cells, and then...

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Problems solved by technology

[0003] For the current stem cell sorting technology, magnetic bead sorting is more common, but in conventional sorting, the magnetic beads will be cultured together with the stem cells, that is, the magnetic beads are not detached from the stem cells. Production will definitely have a certain impact. Although there are some methods, such as adding some bacteria or other substances for degradation, this is equivalent to adding other components such as exogenous proteins, causing secondary pollution to stem cells, so in fact did not solve the above problems

[0004] There are also some reports on the extraction and purification of stem cell culture supernatant, but the effect is still not good. For example, the patent with the notification number CN102600057B discloses a preparation method of human placental stem cell extract freeze-dried powder. A single 3000D filter membrane is used for ultrafiltration interception, which removes some impurities, but the impurities such as sugars are still not removed, and it also leads to a large loss of cytokines

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