Application of 1,6-hexanediol or derivatives thereof in preparation of hematologic tumor resistant drugs
A technology of tumor drugs and derivatives, which is applied in the direction of anti-tumor drugs, drug combinations, active ingredients of hydroxyl compounds, etc., to achieve the effect of inhibiting the liquid phase separation process, good application prospects, and inhibiting occurrence
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[0021] 1. Experimental materials
[0022] 1,6-Hexanediol, supplier Sigma;
[0023] RPMI medium, English name RPMI Medium Modified, supplier HyClone;
[0024] PBS(1×), supplier HyClone;
[0025] Penicillin-Streptomycin Solution, English name Penicillin-Streptomycin Solution, supplier HyClone;
[0026] Fetal bovine serum, supplier Hangzhou Sijiqing Biological Engineering Materials Co., Ltd.;
[0027] CCK-8, English name Cell Counting Kit-8, supplier Dojindo Laboratories;
[0028] PI, English name Propidium Iodide, supplier Sigma.
[0029] 2. Experimental method
[0030] 1. 1,6-Hexanediol solution and PI dyeing solution configuration
[0031] 1,6-Hexanediol powder is dissolved in sterile water, the storage concentration is 1g / mL, and it is stored at 4℃ and protected from light;
[0032] PI was dissolved in PBS at a storage concentration of 10 mg / mL, and stored at 4°C in the dark.
[0033] 2. Cell culture
[0034] Diffuse large B-cell lymphoma cells HBL-1, SUDHL-2, myeloma cells U266, and Hodgkin’s...
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