Duchenne muscular dystrophy (DMD) detection gene chip and kit and use method of kit

A muscular dystrophy and gene chip technology, which is applied in the field of detection of pseudohypertrophic muscular dystrophy, can solve the problems of false positives and easy contamination of samples, and achieve the effects of high specificity, shortened detection time, and high repeatability

Inactive Publication Date: 2017-12-01
MATERNAL & CHILD HEALTH HOSPITAL OF GUANGXI ZHUANG AUTONOMOUS REGION GUANGXI ZHUANG AUTONOMOUS REGION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, MLPA technology has certain limitations, such as the need to accurately measure the concentration of DNA, the sample is easily contaminated, and there is a certain possibility of false positives, especially for the case of single exon d

Method used

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  • Duchenne muscular dystrophy (DMD) detection gene chip and kit and use method of kit
  • Duchenne muscular dystrophy (DMD) detection gene chip and kit and use method of kit
  • Duchenne muscular dystrophy (DMD) detection gene chip and kit and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Embodiment, content is as follows:

[0019] The gene chip comprises a solid phase carrier and oligonucleotide probes immobilized on the solid phase carrier, wherein the oligonucleotide probe is the nucleotide sequence shown in SEQ ID NO.1-345; the solid phase carrier is the Glass slides, silicon wafers, nitrocellulose, nylon, or polystyrene. The gene chip is included in the kit. In addition, the kit also includes enzyme cutting reagents, markers, labeled product purification columns, hybridization solution, and washing solution. The oligonucleotide probes in this example were produced by Agilent Technologies Co., Ltd., and the gene chip was also produced by Agilent Technologies Co., Ltd. according to conventional methods.

[0020] How to use, the steps are as follows:

[0021] (1) Preparation of sample DNA fragments:

[0022] The patient's blood was taken, and the whole genome DNA (gDNA) was extracted by conventional methods. Thaw 10×Restriction EnzymeBuffer and BSA...

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PUM

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Abstract

The invention discloses a duchenne muscular dystrophy (DMD) detection gene chip and kit and a use method of the kit. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, and the oligonucleotide probe is indicated in a nucleotide sequence of SEQ ID No. 1-345; the gene chip is contained in the kit; the use method of the kit includes the steps of a), preparing sample DNA fragments; b), fluorescently marking the DNA fragments; c), purifying gDNA markers; d), allowing the gDNA markers to be interbred with the gene chip; e), cleaning the interbred gene chip and scanning chip interbreeding signals to acquire results. The gene chip and the kit are low in cost, simple in detection operations, high in specificity and good in stability, the process from sample extraction to acquisition of the scanning results can be completed within two days, and the gene chip and the kit are suitable for detection for one or multiple exon deletion or repeat mutations in clinical patient DMA gene and antenatal diagnosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a gene chip for detecting pseudohypertrophic muscular dystrophy, a kit and a use method thereof. Background technique [0002] Pseudohypertrophic muscular dystrophy is a genetic disorder characterized by progressive muscle weakness and atrophy, and is the most common X-linked recessive, hereditary muscle wasting disorder, including Duchenne progressive muscular dystrophy (DMD) and Bayer's progressive muscular dystrophy Muscular dystrophy BMD, the overall incidence of the two is about 1 / 3500-1 / 5000 live-born male babies, both caused by DMD gene mutations. The dystrophin protein encoded by the DMD gene is mainly located in skeletal muscle and cardiac muscle, and has the function of protecting and stabilizing muscle fibers, and also has the function of intracellular chemical signal transduction. [0003] DMD patients generally have no obvious abnormalities after birth, and most childre...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C40B40/06
CPCC12Q1/6837C12Q2565/501C12Q2563/107
Inventor 沈亦平张淑杰覃海松何升陈碧艳
Owner MATERNAL & CHILD HEALTH HOSPITAL OF GUANGXI ZHUANG AUTONOMOUS REGION GUANGXI ZHUANG AUTONOMOUS REGION
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