Device and method for detecting fluorescent micro ribonucleic acid (RNA)

Abstract

The invention relates to a device and a method for detecting fluorescent micro ribonucleic acid (RNA), belonging to the technical field of biological detection. The device is characterized in that an excitation light source A is positioned at the left side of a sample tank, and an excitation light source B is positiond at the right side of the sample tank; the excitation light source A and the excitation light source B are respectively welded on a circuit board, embedded in an optical transmission channel and used for exciting fluorescent substances in a sample; an excitation light detection unit A is positioned between the excitation light source A and the sample tank, and an excitation light detection unit B is positioned between the excitation light source B and the sample tank; the heights of the excitation light detection unit B and the sample tank are lower than that of the excitation light source B; the excitation light detection unit B and the sample tank are welded on the circuit board and embedded in the optical transmission channel, so that excitation light is enabled to irradiate the sample in the sample tank. The device and the method for detecting the fluorescent micro RNA have the beneficial effects that a fluorescence intensity detection unit is formed by a band-pass filter and a photodiode, so that the switch between two types of excitation light is realized; an optical transmission channel module is additionally arranged, so that a whole optical detection system can be isolated and protected, and the accuracy and sensitivity of an experiment are improved.

Description

technical field [0001] The invention belongs to the technical field of biological detection. Background technique [0002] Gene (DNA or RNA) is the carrier of genetic information and plays an extremely important role in the life processes of individual growth, development, reproduction, heredity and variation. At present, the RNA quantitative methods commonly used in medicine mainly include ultraviolet spectrophotometry (260nm), fluorescent quantitative PCR technology and so on. However, UV spectrophotometry is often interfered by DNA and protein, the specificity of quantification is poor, the sensitivity is lower than that of fluorescence detection, and the sample volume that needs to be consumed is large, etc., and the specific quantification of RNA cannot be achieved. Fluorescent quantitative PCR technology is a new technology developed in recent years. It has the characteristics of rapid quantification, strong specificity, and high sensitivity. However, it is expensive ...

AI Technical Summary

Problems solved by technology

However, UV spectrophotometry is often interfered by DNA and protein, the specificity of quantification is poor, the sensitivity is relatively low compared with fluorescence detection, and the sample volume required to be consumed is large, etc., and the specific quantification of RNA cannot be achieved.

Fluorescent quantitative PCR technology is a new technology developed in recent years. It has the characteristics of rapid quantification, strong specificity, and high sensitivity. However, it is expensive and the quantitative results are easily affected by the quality of sample RNA, which objectively limits the miniaturization of the instrument. and portability

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