Line light source excitation-based rapid high-throughput hyperspectral detection device and detection method
A detection device and detection method technology, applied in the field of hyperspectral detection, can solve problems such as limited spatial resolution, difficult quantitative analysis of objects, and large impact, and achieve high signal-to-noise ratio, low loss of optical components and extended life Effect
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Embodiment 1
[0037] Combine below figure 1 Be explained. Detect unstained tissue sections to assist in judging cancerous tissues and their borders. At this time, the wavelength of the push-broom imaging spectrometer used is a near-infrared imaging spectrometer. The wide-spectrum light source 1 is focused on the slice of the tissue sample 4 to be tested through the objective lens or the lens 2 , and the slice of the sample 4 to be tested is fixed on the translation stage 3 . The one-dimensional scanning of the slice of the sample 4 to be tested is realized by the movement of the displacement stage 3 . The transmitted light passes through the filter 5 and the focusing lens 6 in turn, and then enters the imaging spectrometer 7, 8 is a computer, and 9 is a drive controller of the translation stage 3. The line light source at this time is produced by focusing a broad-spectrum light source onto a slit.
[0038] The y of the sample coordinates corresponds to the y1 of the area array camera, a...
Embodiment 2
[0043] The optical path structure of the system is as figure 2 shown. figure 2 Among them, 1 is the excitation line light source, 3 is the translation stage, 4 is the sample to be tested, 5 is the filter, 6 is the focusing lens, 7 is the imaging spectrometer, 9 is the controller of the translation stage, 8 is the computer, and 10 is the two-way The color mirror, 11 is a lens, and the computer controls the data acquisition of the imaging spectrometer and the movement of the displacement stage. The light emitted by the line light source 1 is reflected by the dichroic mirror 10 , and then focused on the slice of the sample 4 to be measured by the lens 11 . The sample 4 to be tested is fixed on the displacement stage 3, and the sample is scanned by controlling the movement of the displacement stage 3; the signal radiated by the sample passes through the focusing lens 11, the dichroic mirror 10, the filter 5, the focusing lens 6, and the focusing into a pushbroom imaging spectr...
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