Low-alkali gel encapsulated microbial self-healing agent and application thereof

A microbial and self-healing technology, applied in the biological field, can solve the problems of limited slag or ceramsite loading, limited repair efficiency, strength loss, etc., to save maintenance funds, improve repair efficiency, and good compatibility.

Active Publication Date: 2017-12-08
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But on the one hand, due to the low strength of slag or ceramsite, its introduction will cause strength loss to the cement-based material matrix; on the other hand, the loading capacity of slag or ceramsite is limited, which limits the repair efficiency

Method used

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  • Low-alkali gel encapsulated microbial self-healing agent and application thereof
  • Low-alkali gel encapsulated microbial self-healing agent and application thereof
  • Low-alkali gel encapsulated microbial self-healing agent and application thereof

Examples

Experimental program
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Embodiment 1

[0028] A low-alkali gelation encapsulated microbial self-repairing agent prepared by the following method. Inoculation of Sarcina pasteurii Sporosarcina pasteurii in a basal medium, and each liter of culture medium contains 3g beef extract, 5g peptone and 18g urea , At 37℃, shake culture at 100 rpm on a water bath shaker, 21 days later, centrifuge the obtained culture broth on a centrifuge at 4000 rpm for 10 min, remove the supernatant and collect the spore liquid with deionized water to concentrate the spore The final concentration of the liquid is controlled at 2×10 9 cell / ml. Prepare beef extract and peptone with a mass ratio of 1:1.6 as well as a low-alkali gelling medium with a mass ratio of sulphoaluminate cement and silica fume of 1:0.2. Then 40g of nutrients and 240g of low-alkali gelling medium are combined with After mixing 100g of water, stir for 5 minutes, then mix with the concentrated spore liquid at a volume ratio of 150:1. Place it at 20℃ and 100% relative humid...

Embodiment 2

[0030] A low-alkali gelation encapsulated microbial self-repairing agent prepared by the following method, inoculating Sarcina pasteurii into a basic medium, and each liter of culture medium contains 2.4g beef extract, 4g peptone and urea 16g, shake culture on a water bath shaker at a speed of 150rpm at 25°C, 14 days later, centrifuge the obtained culture broth on a centrifuge at 3000rpm for 20min, remove the supernatant and collect the spore broth with deionized water to concentrate the spores The final concentration of the bacterial liquid is controlled at 2×10 8 cell / ml. Prepare beef extract and peptone with a mass ratio of 1:2, and a low-alkali gelling medium with a mass ratio of sulphoaluminate cement and silica fume of 1:0.1, and then combine 36g of nutrients, 220g of low-alkali gelling medium with After mixing 100g of water, stir for 3min, then mix with the concentrated spore liquid at a volume ratio of 100:1. Place it in an environment of 20℃ and 100% relative humidity ...

Embodiment 3

[0032] A low-alkali gelation encapsulated microbial self-repairing agent prepared by the following method. Inoculation of Sarcina Pastoris Sporosarcina pasteurii in a basic medium, and each liter of culture medium contains 3.6 g of beef extract, 6 g of peptone and urea 20g, shake culture at 30°C on a water bath shaker at a speed of 120rpm, 28 days later, centrifuge the obtained culture broth on a centrifuge at 4000rpm for 15min, remove the supernatant and collect the spore broth with deionized water to concentrate the spores The final concentration of the bacterial liquid is controlled at 2×10 10 cell / ml. Prepare beef extract and peptone with a mass ratio of 1:1.8 and a low-alkali gelling medium with a mass ratio of sulphoaluminate cement and silica fume of 1:0.15. Then, 40g of nutrients, 230g of low-alkali gelling medium and After mixing 100g of water, stir for 5 minutes, then mix with the concentrated spore liquid at a volume ratio of 100:1. Place it at 22°C and 100% relative...

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Abstract

The invention relates to a low-alkali gel encapsulated microbial self-healing agent and application thereof. The self-healing agent comprises a component A and a component B, wherein the component A is obtained by mixing and stirring a nutritional ingredient, a low-alkali gel medium and water, uniformly mixing a mixed solution and a concentrated bacillus solution, standing for hardening, and grinding to form particles; the component B is a mixture of urea and anhydrous calcium nitrate. Dry-mixed cement mortar with the added microbial self-healing agent is used for self-healing of gaps of cement, bacterial cells can be effectively protected, the ore yield is increased, high compatibility with a cement base material is achieved, a produced healing medium is natural carbonate, and has high durability, volume stability and environment friendliness, maintenance funds can be greatly saved by a self-healing characteristic, meanwhile, the loading capacity can be remarkably improved, moreover, high compatibility with the cement base material is achieved, the influence on own performance of the cement base material can be avoided, and the self-healing efficiency can also be greatly improved.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-alkali gelation encapsulation type microbial self-repairing agent and its application. Background technique [0002] Biomineralization is a common phenomenon in nature, and the mineralization of microorganisms is a typical representative among them. When the environment meets certain physical and chemical conditions, microorganisms can convert soluble ions into solid minerals through active or passive means, which can selectively fill or bond permeable pores or cracks. It is used in petroleum engineering and geology. The application in engineering and civil engineering has attracted widespread attention. Nearly two-thirds of the more than 30 biological minerals that have been discovered are calcium ore, of which calcite is the most important. As a stable mineral, calcite produced by biomineralization can be used for the self-repair of cement-based materials and other a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B28/06
CPCC04B28/06C04B2111/343C04B24/14C04B24/126C04B22/085C04B2103/0001
Inventor 徐晶王彬彬王先志左俊卿
Owner TONGJI UNIV
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