Detecting kit for polymorphism of warfarin pharmacogenetics genes CYP2C9 and VKORC1

A technology of polymorphism detection and genetics, applied in the field of genetic testing and diagnosis, to achieve good specificity, high sensitivity, and easy to use

Inactive Publication Date: 2017-12-08
苏州康吉诊断试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, recent studies have found that there is a close relationship between the VKORC1-1173C/T gene polymorphism a

Method used

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  • Detecting kit for polymorphism of warfarin pharmacogenetics genes CYP2C9 and VKORC1
  • Detecting kit for polymorphism of warfarin pharmacogenetics genes CYP2C9 and VKORC1

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Embodiment Construction

[0017] The kit for predicting the risk of nasopharyngeal carcinoma described in this embodiment is a kit for predicting the risk of nasopharyngeal carcinoma by detecting the above two SNP sites and EBV typing information.

[0018] The kit of the present invention comprises: PCR amplification primers and single base extension primers for detecting two SNP sites of the human genome, the two SNP sites are rs1057910 and rs9923231;

[0019] One, involving the synthesis of primary PCR amplification primers and extension primers and probe sequences:

[0020] 1. Design and synthesize primary PCR amplification primers and extension primers of the SNP site rs1057910:

[0021] SEQ ID NO: 1 GCTTGAGGGGCTTTAGAGTTG;

[0022] SEQ ID NO: 2 AGGGTTGACGGACGTTGGGTT;

[0023] Secondary PCR amplification primers and extension primers:

[0024] SEQ ID NO: 3 AGAAGGCGTAGAGCATGTCCAG;

[0025] SEQ ID NO: 4GAGTACGACTGTGAGGTGGGCG.

[0026] The probe sequences are as follows:

[0027] SEQ ID NO:5 CATC...

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Abstract

The invention discloses a detecting kit for polymorphism of warfarin pharmacogenetics genes CYP2C9 and VKORC1, belongs to the technical field of genetic test diagnosis, and is used for auxiliary determination of warfarin dosage of a patient. The kit disclosed by the invention comprises specific primers and specific probes which are used for detecting mutations at the sites rs1057910 and rs9923231; the specific primers comprise upstream primers and downstream primers; the specific probes comprise a positive probe and a negative probe; the specific primers and the specific probes of the rs1057910 are respectively the upstream primers as shown in SEQ ID NO.1 and SEQ ID NO.3, the downstream primers as shown in SEQ ID NO.2 and the SEQ ID NO.4, the positive specific fluorescent probe as shown in SEQ ID NO.5, and a negative specific fluorescent probe as shown in SEQ ID NO.6; and the detecting kit for the polymorphism of the warfarin pharmacogenetics genes CYP2C9 and VKORC1 can be used for individualized treatment of warfarin according to genetic genes, and has important significance in subsequent research.

Description

technical field [0001] The invention relates to a kit for detecting polymorphisms of warfarin pharmacogenetic genes CYP2C9 and VKORC1, which belongs to the technical field of gene testing and diagnosis and is used for assisting in determining the dosage of warfarin for patients. Background technique [0002] Warfarin is a coumarin-based oral anticoagulant commonly used in artificial valve replacement, thromboembolic diseases (such as pulmonary embolism) and anticoagulant treatment of atrial fibrillation. Due to the narrow therapeutic window of warfarin, in the course of clinical use, the stable therapeutic dose varies among different individuals. If the dose is insufficient, it will lead to thrombosis. At the same time, if the dose is too large, it will increase bleeding. risk, even life-threatening. Therefore, choosing an appropriate anticoagulant regimen for different individuals has become one of the important factors affecting the long-term efficacy of patients after ca...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16
Inventor 邵敏华黄迅威
Owner 苏州康吉诊断试剂有限公司
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