Traditional Chinese medicine composition and applications thereof
A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition containing various plant polysaccharides and vegetable oils, to achieve the effect of improving the immune function of the body, restoring the immune monitoring function, and reducing the expression rate of positive cells
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Embodiment 1
[0030] Embodiment 1, the preparation of Ganoderma lucidum polysaccharide
[0031] The Ganoderma lucidum polysaccharide of the present invention is prepared by the following preparation method: after crushing the Ganoderma lucidum, add water according to the weight ratio of Ganoderma lucidum: water = 1:10, heat and stir at 70°C for 2 hours, and filter the obtained filter residue according to the above-mentioned ratio of solid to liquid, Continue to extract twice at temperature and time, combine three extractions, concentrate under reduced pressure and centrifuge to obtain a clarified concentrate, add 7 times the volume of 95% ethanol to the clarified concentrate according to the volume ratio of clarified concentrate: 95% ethanol=1:7 After mixing, let it stand overnight at 4°C to precipitate, centrifuge to collect the precipitate, and vacuum freeze-dry to obtain Ganoderma lucidum polysaccharide.
Embodiment 2
[0032] Embodiment 2, the preparation of Morinda officinalis polysaccharide
[0033]The Morinda officinalis polysaccharide of the present invention is prepared by the following preparation method: after crushing Morinda officinalis, add water according to the weight ratio of Morinda officinalis: water = 1:5, heat and stir at 75°C for 3 hours, and filter the obtained filter residue Continue to extract twice according to the above-mentioned solid-liquid ratio, temperature and time, combine the extracts three times, concentrate under reduced pressure and centrifuge to obtain a clarified concentrate, according to the volume ratio of the clarified concentrate: 95% ethanol=1:9, 9 times the volume of 95 % ethanol was added to the clarified concentrated solution, mixed evenly, left to settle overnight at 4°C, collected by centrifugation, and vacuum freeze-dried to obtain the Morinda officinalis polysaccharide.
Embodiment 3
[0034] Embodiment 3, the preparation of polygonatum polysaccharide
[0035] Polygonatum polysaccharides described in the present invention are prepared by the following preparation method: after pulverizing Polygonatum, adding water according to the weight ratio of Polygonatum:water=1:5, heating and stirring at 75°C for 3 hours, and filtering the obtained filter residue according to the above-mentioned ratio of solid to liquid, Continue to extract twice at temperature and time, combine three extractions, concentrate under reduced pressure and centrifuge to obtain a clarified concentrate, add 9 times the volume of 95% ethanol to the clarified concentrate according to the volume ratio of clarified concentrate: 95% ethanol=1:9 Solution, after mixing, let it stand overnight at 4°C to precipitate, centrifuge to collect the precipitate, and freeze-dry in a vacuum to obtain Polygonatum polysaccharide.
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