Metal organic frame material signal amplification electrochemical analysis paper chip sensor

A metal organic framework, electrochemical analysis technology, applied in the field of electrochemical analysis and detection, can solve the problems of no signal amplification, poor conductivity of paper chips, etc., and achieve the effects of reducing preparation cost, low detection limit, and simplifying preparation steps

Inactive Publication Date: 2017-12-15
UNIV OF JINAN
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Problems solved by technology

However, individual paper chips conduct electricity very poorly and do not provide signal amplification

Method used

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  • Metal organic frame material signal amplification electrochemical analysis paper chip sensor
  • Metal organic frame material signal amplification electrochemical analysis paper chip sensor
  • Metal organic frame material signal amplification electrochemical analysis paper chip sensor

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Embodiment example 1

[0033] A metal-organic framework material signal amplification electrochemical analysis paper chip sensor and its application in microRNA detection:

[0034](1) Use Adobe illustrator CS6 on the computer to design the pattern of the hydrophobic area of ​​the paper chip. The hydrophilic area of ​​the paper device includes the detection area (6 mm in diameter) and the auxiliary area (8 mm in diameter), as shown in the attached figure 1 shown;

[0035] (2) Using NaBH 4 Prepare a suspension of gold nanoseeds as a reducing agent, sodium citrate as a stabilizer; prepare freshly prepared growth solution: 1.2 mmol L -1 HAuCl 4 , 2.0 mmol·L -1 Hexadecyltrimethylammonium chloride, 7.2mmol·L -1 h 2 o 2 and 10 mmol·L -1 pH = 7.0 phosphate buffer solution; 15.0 μL of gold nano-seed growth solution was dropped on the surface of the paper fiber in the detection area, incubated at room temperature for 10 minutes, and the resulting modified gold nano-paper electrode was washed 5 times...

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Abstract

The invention discloses a metal organic frame material signal amplification electrochemical analysis paper chip sensor and a method thereof for measuring microRNA. The method comprises the following steps: preparing a paper chip three-electrode system of which the surface is modified by nanogold particles; starting a chain hybridization reaction by adding microRNA while modifying the electrode surface with a functionalized metal organic frame material; dropwise adding a glucose-containing buffer solution in the detection area; through a catalytic reaction between the functionalized metal organic frame material and the glucose, realizing signal amplification and high-sensitivity detection of microRNA by a differential pulse voltammetry.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis and detection, more specifically to the construction of a paper chip laboratory technology platform for metal organic framework material signal amplification electrochemical analysis. Background technique [0002] In recent years, the morbidity and mortality of cancer in my country have shown a gradual upward trend. The occurrence of tumors seriously affects people's quality of life and endangers people's life safety. Accurate, fast and simple detection of microRNA and its physiological activities in tumor cells Detection is of great significance for the diagnosis and treatment of diseases. Therefore, it is very important to find a rapid, quantitative and sensitive method for detecting microRNA in the early detection and treatment monitoring of cancer. The common way of clinical diagnosis is mainly through the detection of relatively expensive large-scale instrument analysis equip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/26
CPCG01N27/26G01N27/30G01N27/3278
Inventor 葛慎光王贺兰飞飞孙晓路鉴燕楠孔庆坤颜梅于京华
Owner UNIV OF JINAN
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