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Porphyrinyl MOFs mimic enzyme as well as preparation method and application thereof

A porphyrin-based, enzyme-mimicking technology, applied in the field of electrochemical analysis, can solve problems such as the instability of biological enzymes, the difficulty of deactivation and purification, and the difficulty of rapid detection by electrochemical DNA sensors, so as to overcome the deactivation and improve anti-interference Power and accuracy, the effect of overcoming the influence of interfering substances

Inactive Publication Date: 2021-07-30
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological enzymes are inherently unstable, easily inactivated, and difficult to purify, making it difficult for electrochemical DNA sensors to be used for on-site rapid detection of heavy metals in fruits and vegetables

Method used

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  • Porphyrinyl MOFs mimic enzyme as well as preparation method and application thereof
  • Porphyrinyl MOFs mimic enzyme as well as preparation method and application thereof
  • Porphyrinyl MOFs mimic enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Disperse 6 mg of zirconium tetrachloride and 5 mg of iron porphyrin into 5 mL of N,N-dimethylformamide, and then add 0.40 mL of acetic acid to it, and then ultrasonically mix. Subsequently, the mixed solution was transferred to a polytetrafluoroethylene reactor and reacted at 120 °C for 24 h. The above reaction solution was cooled to room temperature, centrifuged at 10,000 r / min for 15 min to obtain a brown precipitate, and vacuum-dried at 60 °C for 12 h to obtain the porphyrin-based MOFs mimetic enzyme.

[0035] figure 1 It is a scanning electron microscope picture of the porphyrin-based MOFs mimic enzyme. It can be seen from the figure that the appearance of the porphyrin-based MOFs mimic enzyme prepared in the present invention is regular and spindle-shaped, with a long axis of about 480 nm and a short axis of about 150 nm. nm. figure 2 It is the infrared spectrum of porphyrin-based MOFs mimic enzymes and epoxy-functionalized MOFs. It can be seen from the figure t...

Embodiment 2

[0037] Disperse 4 mg of zirconium tetrachloride and 3 mg of iron porphyrin into 2 mL of dimethylformamide, then add 0.20 mL of acetic acid to it, and then ultrasonically mix. Subsequently, the mixed solution was transferred to a polytetrafluoroethylene reactor and reacted at 120 °C for 24 h. The above reaction solution was cooled to room temperature, centrifuged at 10,000 r / min for 15 min to obtain a brown precipitate, and vacuum-dried at 60 °C for 12 h to obtain the porphyrin-based MOFs mimetic enzyme.

Embodiment 3

[0039] Disperse 9 mg of zirconium tetrachloride and 6 mg of iron porphyrin into 10 mL of N,N-dimethylformamide, and then add 1 mL of acetic acid to it and mix well by ultrasonic. Subsequently, the mixed solution was transferred to a polytetrafluoroethylene reactor and reacted at 120 °C for 24 h. The above reaction solution was cooled to room temperature, centrifuged at 10,000 r / min for 15 min to obtain a brown precipitate, and vacuum-dried at 60 °C for 12 h to obtain the porphyrin-based MOFs mimetic enzyme.

[0040] image 3Cyclic voltammograms of mesoporphyrin-based MOFs mimic enzymes, a is without glucose, b is with glucose. It can be seen from the figure that curve a and curve b are the cyclic voltammograms of MOFs mimic enzymes in the presence or absence of hydroxylamine, curve a has no obvious oxidation / reduction peak, while curve b has an obvious reduction at 0.28 V peak, indicating that the MOFs mimic enzyme has obvious catalytic properties for hydroxylamine.

[0041...

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Abstract

The invention provides porphyrinyl MOFs mimic enzyme as well as a preparation method and application thereof, and belongs to the technical field of electrochemical analysis. In the invention, the porphyrinyl MOFs mimic enzyme with good stability and high catalytic activity is prepared, and then an electrochemical DNA sensor based on the porphyrinyl MOFs mimic enzyme is constructed, and the electrochemical DNA sensor can sensitively, accurately and quickly detect heavy metal ions in fruit and vegetable juice.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis, and in particular relates to a porphyrin-based MOFs mimic enzyme and a preparation method and application thereof. Background technique [0002] Fruits and vegetables can supplement the human body with various vitamins and minerals, and enhance cell vitality and gastrointestinal function, so they are favored by consumers. However, fruits and vegetables are extremely susceptible to heavy metal pollution during the growth process, which affects the edible safety of fruit and vegetable juices. Therefore, it is particularly important to research and develop sensitive, rapid and accurate detection methods for heavy metals in fruit and vegetable juices. [0003] Traditional heavy metal detection methods mainly include atomic absorption spectrometry, inductively coupled plasma mass spectrometry, and liquid chromatography. However, most of the above methods have disadvantages such as c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00G01N27/30G01N27/48
CPCC08G83/008G01N27/308G01N27/48
Inventor 张新爱石吉勇邹小波黄晓玮李志华张迪申婷婷张俊俊李文亭
Owner JIANGSU UNIV
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