Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Whole-set primer for detecting polymorphism of rs11190870 in human genome and application of whole-set primer

A technology for genome and human detection, which is applied to the detection of the polymorphism of rs11190870 in the human genome and its application field. It can solve the problems of poor detection timeliness, a burden of detection cost, and long detection cycle, so as to reduce the detection cost, The effect of shortening the inspection cycle

Inactive Publication Date: 2017-12-26
北京东方亚美基因科技研究院有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem brought by this outbound sequencing method is that the detection timeliness is poor, and the detection cycle is usually long
At the same time, although the price of Sanger sequencing for a single sample is currently low, the cost of testing is still a burden for laboratories with large samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Whole-set primer for detecting polymorphism of rs11190870 in human genome and application of whole-set primer
  • Whole-set primer for detecting polymorphism of rs11190870 in human genome and application of whole-set primer
  • Whole-set primer for detecting polymorphism of rs11190870 in human genome and application of whole-set primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Detection of the polymorphism of rs11190870 in the human genome (genotyping detection of rs11190870 locus)

[0027] 1. Design of primers for four-primer variant retardation amplification system

[0028] Download the sequence corresponding to rs11190870 from the NCBI database, the upstream and downstream of the SNP site are 500 bp each, and the total length is 1001 bp. The rs11190870 genotype includes TT, TC, and CC. Primers were designed through the design website provided by Ye et al. The website URL is: http: / / primer1.soton.ac.uk / primer1.html . The primers designed by the software are named primer set A, and the primer set A is composed of the following AIS_Fin, AIS_Rin, AIS_F and AIS_R:

[0029] ① Internal primer 1, the primer corresponding to the T allele, named AIS_Fin, the sequence is:

[0030] 5'-AGGAATTATCAACTAGAATTTGATTAAGAT-3' (SEQ ID No. 1).

[0031] ②Inner primer 2, the primer corresponding to the C allele, named AIS_Rin, the sequence is:

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a whole-set primer for detecting polymorphism of rs11190870 in a human genome and application of the whole-set primer. The whole-set primer for detecting the polymorphism of rs11190870 in the human genome consists of four primers which are respectively named as AIS_Fin, AIS_Rin2, AIS_F and AIS_R, wherein AIS_Fin is single stranded DNA of SEQ ID No.1; AIS_Rin2 is single stranded DNA of SEQ ID No.2; AIS_F is single stranded DNA of SEQ ID No.3; and AIS_R is single stranded DNA of SEQ ID No.4. Compared with Sanger detection, the whole-set primer is capable of shorting the detection period and lowering the detection cost and can be applied to screening and prevention of AIS high risk groups and idiopathic scoliosis of teenagers.

Description

technical field [0001] The invention relates to a set of primers for detecting polymorphism of rs11190870 in human genome and application thereof in the field of biotechnology. Background technique [0002] Scoliosis, also known as scoliosis, is a spinal lesion that mostly occurs in adolescents and seriously affects the physical and mental health of adolescents. Scoliosis is a disease that develops for a long time. Once it occurs, it will gradually develop and worsen with the growth and development of adolescents. It will also lead to more difficult treatment and worse treatment effect. Therefore, early detection and intervention of scoliosis are particularly important. At present, the most commonly used method for the screening of scoliosis in adolescents is on-site screening to intervene and treat those with scoliosis already detected. However, due to the small degree of scoliosis in the early stage of scoliosis, it is not easy to be detected, so some patients with early...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2531/113C12Q2535/137
Inventor 张少鹏郭金海曾沃坦
Owner 北京东方亚美基因科技研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com