The invention discloses a preparation method of a zebra fish model for teenager
idiopathic scoliosis. The preparation method comprises the following steps: S1, synthesizing RNP; s2, injecting RNP into a wild zebra
fish embryo within 1 hour of birth to obtain an F0
embryo; s3, carrying out PCR detection on the
mutation condition of the
target site; s4, screening
zebrafish genetic lines with
stable gene knockout; s5, selecting
tail-rolling malformed
juvenile fishes, feeding the
juvenile fishes until the
juvenile fishes are adult, and regularly observing phenotypes; s6, extracting
DNA from the adult zebra fish, and detecting to confirm that
gene editing succeeds; and S7, confirming successful establishment of the model according to
a DNA sequencing result and zebra fish lateral convex
phenotype, belonging to the technical field of animal models, and starting from a positive result of clinical
genetics screening, enabling the model to have the representativeness of the
disease; a
gene editing method is adopted,
mutation is edited into a zebra fish
genome, a Cas9
gene editing technology is used for experiment, the gene editing efficiency is ensured, and the positive rate of the constructed lateral convex model is high.