Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Applications of IncRNA in preparation of products which are used for diagnosis or prediction of adolescent idiopathic scoliosis

A scoliosis and idiopathic technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as inconsistent conclusions, inability to explain the occurrence of AIS, progress, etc., to achieve Effect of early treatment saving, medical cost saving

Active Publication Date: 2018-06-08
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of researches on its genomics and proteomics at home and abroad have achieved certain results, the conclusions are not consistent, and it is impossible to make a satisfactory explanation for the occurrence and progress of AIS.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Applications of IncRNA in preparation of products which are used for diagnosis or prediction of adolescent idiopathic scoliosis
  • Applications of IncRNA in preparation of products which are used for diagnosis or prediction of adolescent idiopathic scoliosis
  • Applications of IncRNA in preparation of products which are used for diagnosis or prediction of adolescent idiopathic scoliosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Sample collection standard

[0039] 1. Research object

[0040] This study adopts a case-control research method. The case group of peripheral blood samples is selected from Chinese Han female AIS who underwent scoliosis correction in Peking Union Medical College Hospital.

[0041] Paraspinal muscle specimens The case group was selected from Chinese Han female AIS patients who underwent scoliosis correction in Peking Union Medical College Hospital, and the control group was the intervertebral disc degeneration surgery patients without spinal deformity matched with the case group in terms of gender, age, and ethnicity.

[0042] 1.1 Case group

[0043] All come from adolescent idiopathic scoliosis patients undergoing surgical treatment in our hospital from April 2013 to March 2014. Complete clinical data and imaging data (including spinal X-ray, CT and three-dimensional reconstruction, total spine MRI) were selected. ) Han adolescent idiopathic scoliosis patient...

Embodiment 2

[0067] The screening of embodiment 2lncRNA

[0068] Four cases of adolescent idiopathic scoliosis patients were taken as the AIS group, and four normal controls were taken as the normal control group (NC group). Fasting peripheral blood was collected and total RNA was extracted.

[0069] 1. Extraction of total RNA from peripheral whole blood

[0070] 1) Add 0.25ml peripheral blood and 0.75mL Trizol LS reagent cryopreservation mixture to each EP tube, shake vigorously to mix;

[0071] 2) Incubate at room temperature for 5 minutes to completely dissociate the nucleic acid-protein complex;

[0072] 3) Add 0.2ml chloroform to each EP tube (that is, add 0.2ml chloroform to every 0.75ml Trizol LS reagent reagent sample), and tightly cap the EP tube. Shake vigorously for 15 seconds;

[0073] 4) Stand at room temperature for 3 minutes, so that the organic phase and the inorganic phase can be separated quickly and effectively;

[0074] 5) Centrifuge at 4°C and 12000G for 10 minutes...

Embodiment 3

[0155] Embodiment 3 qRT-PCR large sample verification

[0156] Taking 20 age-matched AIS patients and 20 normal females as the research objects, ENST00000414894.1 and TRHDE-AS1, which were significantly differentially expressed and predicted to be involved in the pathogenesis of AIS, were selected for real-time quantitative PCR verification.

[0157] 1. Extraction of total RNA from peripheral whole blood

[0158] The steps are shown in Example 2.

[0159] 2. The reverse transcription process of cDNA synthesis:

[0160] Reverse transcribe the qualified RNA into cDNA, the specific steps are as follows:

[0161] 1) Removal of DNA in the RNA sample: add the reagents in Table 8 below to the centrifuge tube in turn, and vortex to mix.

[0162] Table 8 Reagents

[0163]

[0164] 2) Slightly centrifuge, place in a PCR instrument, and react at 42°C for 5 minutes;

[0165] 3) Prepare the reverse transcription system as shown in Table 9, add it to the centrifuge tube that has com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses novel molecular markers ENST00000414894.1 and / or TRHDE-AS1 which are related to adolescent idiopathic scoliosis. It is confirmed that expression down-regulate of ENST00000414894.1 and TRHDE-AS1IncRNA in patients with adolescent idiopathic scoliosis is induced, so that applications in preparation of products which are used for diagnosis or prediction of adolescent idiopathicscoliosis are realized. The invention also discloses applications of the molecular markers in preparation of kits which are used for diagnosis or prediction of adolescent idiopathic scoliosis. The molecular markers can be used for rapid and effective diagnosis of early stage adolescent idiopathic scoliosis. Significant importance on early stage treatment and chemical cost reduction of adolescentidiopathic scoliosis is achieved, and treatment targets and important basis are provided for clinical applications such as gene treatment and medicine treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of ENST00000414894.1 and / or TRHDE-AS1 in the preparation of products for diagnosing or predicting juvenile idiopathic scoliosis. Background technique [0002] Idiopathic scoliosis (Idiopathic Scoliosis, IS) is a disease with a high incidence rate (1.3%) and seriously affects the quality of life, accounting for about 80% of the total incidence of scoliosis. According to the age of onset, it is divided into infantile idiopathic scoliosis (Infantile Idiopathic Scoliosis, IIS) (0.3 years old), juvenile idiopathic scoliosis (Juvenile Idiopathic Scoliosis, JIS) (3-9 years old), adolescent idiopathic scoliosis Adolescent Idiopathic Scoliosis (AIS) (10-16 years old). Among them, AIS accounts for about 80% of the patients with idiopathic scoliosis. The etiology of AIS is not yet clear, and there are many hypotheses describing its pathogenesis, but none of them can explain th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 王以朋李子全李政垚刘书中王牧川牛潼
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com