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A method for rapidly detecting the single nucleotide polymorphism of cattle crabp2 gene and its special kit

A technology of single nucleotide polymorphism and single nucleotide mutation, applied in the field of molecular genetics

Inactive Publication Date: 2020-01-10
NORTHWEST A & F UNIV
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Problems solved by technology

However, CRABP2 transports RA into the nucleus to combine with RAR:RXR heterodimers, causing subsequent RA-induced gene transcription. It has been found that abnormal regulation of CRABP2 exists in many different cancers, and for livestock CRABP2 gene mononuclear The research on nucleotide polymorphism has not been reported yet

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  • A method for rapidly detecting the single nucleotide polymorphism of cattle crabp2 gene and its special kit
  • A method for rapidly detecting the single nucleotide polymorphism of cattle crabp2 gene and its special kit
  • A method for rapidly detecting the single nucleotide polymorphism of cattle crabp2 gene and its special kit

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Embodiment Construction

[0033] The present invention utilizes the PCR-RFLP method to detect the single nucleotide polymorphisms at positions 2458 and 3878 of the cattle CRABP2 gene. The present invention is described in detail below, which is an explanation rather than a limitation of the present invention.

[0034] The results of the resequencing of the cattle genome, transcriptome sequencing and DNA methylome sequencing in the laboratory showed that there are polymorphisms in the cattle CRABP2 gene, and mRNA and DNA methylation in the muscle tissue of Qinchuan cattle fetal and adult cattle There are differences in levels. Therefore, it is speculated that this gene has a regulatory effect on the growth and development of cattle, and it is very important to study the single nucleotide polymorphism of the CRABP2 gene in cattle. As a molecular marker for assisted selection in molecular breeding of cattle, it can provide theoretical basis for molecular breeding of cattle.

[0035] 1. Cloning and polymorp...

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Abstract

The invention discloses a method and special kit for rapidly detecting single nucleotide polymorphism of a cattle CRABP2 gene. The method comprises the following steps: carrying out PCR amplification on the cattle CRABP2 gene by taking cattle whole genome DNA as a template and primer pairs P1 and P2 as primers; after a PCR product is digested by virtue of restriction endonucleases ApaI and Sal I, respectively, carrying out agarose gel electrophoresis; and identifying the single nucleotide polymorphism of a site 2458 and a site 2878 of the cattle CRABP2 gene. According to the method, the single nucleotide polymorphism of the cattle CRABP2 gene can be detected, and relevant molecular genetic markers close to the growth character of the cattle are screened and detected from a DNA level and are applied to the assistant selection and molecular breeding of the cattle, so that the stock breeding speed of the local cattle is increased.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the screening and detection of cattle gene single nucleotide polymorphism (SNP) as molecular genetic marker, and particularly relates to a method and application for detecting cattle CRAABP2 gene single nucleotide polymorphism. Background technique [0002] With the development of modern molecular biology and molecular cloning technology, genetic markers have been developed from morphological markers, cell markers, biochemical markers to DNA molecular markers. DNA molecular markers can directly reflect the differences at the molecular level of DNA. In addition, their polymorphisms are high and the number is large, and the markers are dominant or co-dominant, so their theoretical and application values ​​are immeasurable. DNA molecular marker-assisted selection breeding is an important aspect of its application, because it is not only accurate and efficient, but also not restricted by ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/683C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 黄永震文逸凡郑立张子敬张桂民徐嘉威李继超雷初朝党瑞华蓝贤勇陈宏
Owner NORTHWEST A & F UNIV
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