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Photoelectrochemical sensor for measuring oxytetracycline and its preparation method and application

A photoelectrochemical and oxytetracycline technology, applied in the field of electrochemistry, can solve problems such as residues, health and environmental safety threats, and achieve excellent performance.

Active Publication Date: 2019-12-13
深圳爱湾医学检验实验室
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Oxytetracycline is a tetracycline antibiotic. Due to its good antibacterial properties and low price, it is abused in veterinary drugs and feed additives, resulting in a large amount of residues in food and natural water bodies, posing a serious threat to human health and environmental safety.

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  • Photoelectrochemical sensor for measuring oxytetracycline and its preparation method and application
  • Photoelectrochemical sensor for measuring oxytetracycline and its preparation method and application
  • Photoelectrochemical sensor for measuring oxytetracycline and its preparation method and application

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with specific experiments.

[0027] experimental method

[0028] 1.1 Preparation of reagents

[0029] 1. OTC-aptamer reaction solution: the concentration of each component is: 100mM NaCl, 20mM Tris-HCl, 5mM KCl, 1mM CaCl 2 ,2mM MgCl 2 ,pH=7.6, sterilized at 121°C for 15min, stored at 4°C.

[0030] 2. OTC-aptamer solution: The chain sequence of OTC-aptamer is: 5'-NH 2 -(CH 2 ) 6 -GGA ATT CGC TAGCAG GTT GAC GTT GAC GCT GGTGCC CGG TTG TGG TGC GAG TGT TGT GTG GAT CCG AGCTCC ACG TG-3', each tube is filled with 1 OD DNA dry powder, first centrifuge it at 12000r for 1min to make the powder To the bottom, add an appropriate volume of buffer to make a stock solution with a concentration of 100 μM, and store it in a -20°C refrigerator. During the experiment, the required concentration was 1.5 μM. The stock solution was diluted with buffer solution (pH=7.4) to obtain the required concentration, and then ...

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Abstract

The invention relates to the technical field of electrochemistry and environment detection, and specifically relates to a photo-electrochemical sensor for measuring terramycin. The sensor is a terramycin aptamer / TiO2-rgo / FTO electrode. The sequence of the terramycin aptamer is represented by SEQ ID No.1. The preparation method comprises the following steps: depositing nano TiO2 on FTO conductive glass at first to obtain a TiO2 / FTO conductive glass electrode; then reducing a graphene oxide solution by an electrochemical method, depositing the reduced graphene oxide solution on the TiO2 / FTO conductive glass electrode to obtain a TiO2-rgo / FTO electrode, and finally adhering a terramycin aptamer on the TiO2-rgo / FTO electrode to obtain the terramycin aptamer / TiO2-rgo / FTO electrode. The photo-electrochemical sensor for measuring terramycin has the advantages of excellent performance, stability, low cost, and strong selectivity, and can detect trace terramycin in a solution.

Description

technical field [0001] The invention relates to the field of electrochemical technology and the field of environmental detection technology, in particular to a photoelectrochemical sensor for detecting oxytetracycline. Background technique [0002] Oxytetracycline is a tetracycline antibiotic. Due to its good antibacterial properties and low price, it is abused in veterinary drugs and feed additives, resulting in a large amount of residues in food and natural water bodies, posing a serious threat to human health and environmental safety. . Therefore, it is necessary to develop a sensitive, specific, rapid and low-cost method for the determination of oxytetracycline residues. [0003] Chinese Patent Publication No. CN 105259231 discloses an electrochemical aptamer electrode for oxytetracycline detection and its preparation method. The aptamer electrode includes a glassy carbon electrode, and the outer surface of the glassy carbon electrode is sequentially attached with Redu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/305G01N27/3278
Inventor 封科军刘丽园解芳冯颖
Owner 深圳爱湾医学检验实验室