Easily disassembled cholera diagnosis device for liquid flow driving through interfacial characterization

Abstract

The invention relates to an easily disassembled cholera diagnosis device for liquid flow driving through interfacial characterization and belongs to the field of analysis testing. A series of difficulties can be resulted if polydimethylsiloxane, namely PDMS, which is low in price and very easy to process, is adopted to manufacture a cholera diagnosis micro-fluidic chip. The scheme of the invention is characterized in that PDMS with an original ecological surface is adopted as a substrate material, the channels of the chip are filled with doped micron-order titanium dioxide granules, super-hydrophilicity properties of the surfaces of the titanium dioxide granules can be induced by preset visible light, sets of super-hydrophilic gaps and channels of the titanium dioxide granules substitute and exceed original super-hydrophilic channels, and thus a constant and super-hydrophilic modification effect is achieved; meanwhile, differences of interfacial tension are induced from the head end and the tail end of the chip by using ultrasonic waves with micro power, and thus a test sample liquid is guided to flow to a terminal direction along the channels of the chip.

Description

technical field [0001] The invention relates to an easily disassembled cholera diagnosis device driven by liquid flow by means of interface characteristics. The device is a special device for diagnosing cholera antigen based on antigen / antibody specific reaction and belongs to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic cholera diagnosis, please refer to CN 200910150930.4 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about the key issues of this case. ...

AI Technical Summary

Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

Images