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Complex inoculant preventing and treating verticillium dahliea wilt on tomato, and preparation method and application of complex inoculant

A compound bacterial agent and Verticillium wilt technology, applied in the field of agricultural biology, can solve problems such as high toxicity, polluted soil, and bacterial resistance

Active Publication Date: 2017-12-29
天津坤禾生物科技集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Crop rotation is not feasible for facility agriculture, and although the application of chemical agents can effectively prevent the occurrence of tomato verticillium wilt, it is highly toxic and seriously pollutes the soil. Long-term use will also cause pathogenic bacteria to develop drug resistance and seriously endanger the ecological balance.
After inquiry, there is currently no report on the use of compound Bacillus microbial agents to control tomato Verticillium wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A compound microbial agent for preventing and treating Verticillium wilt of tomato, said composite microbial agent is composed of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:3:4;

[0053] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0054] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganism Culture Preservation Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoy...

Embodiment 2

[0088] A compound microbial agent for preventing and treating Verticillium wilt of tomato, said composite microbial agent is composed of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:2:3;

[0089] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0090] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganism Culture Preservation Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoy...

Embodiment 3

[0124] A compound microbial agent for preventing and treating Verticillium wilt of tomato, said composite microbial agent is composed of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:2:4;

[0125] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0126] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganism Culture Preservation Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoy...

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PUM

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Abstract

The invention relates to a complex inoculant preventing and treating verticillium dahliea wilt on a tomato, and a preparation method and an application of the complex inoculant. The complex inoculant is formed by mixing a fermentation product of bacillus amyloliquefaciens MES812, a fermentation product of bacillus subtilis MES810 and a fermentation product of bacillus mucilaginosus MES803 at a volume ratio of 1:(1-3):(1-5). The preparation method of a strain in the complex inoculant comprises the following steps of strain activation, primary seed solution preparation, secondary seed solution preparation, fermentation tank fermentation and discharge to form the fermentation product. Major microorganisms in the complex inoculant are reproduced extensively in soil to antagonize growth of pathogenic bacteria; harmful microorganisms in the soil are effectively inhibited or killed; a powerful environment is provided for reproduction and growth of beneficial microorganisms; and the occurrence of the verticillium dahliea wilt on the tomato is prevented and controlled effectively by continuous formation of metabolic products of the beneficial microorganisms. At the same time, the soil is improved, the soil fertility is improved, and the output and quality of the tomato are improved.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and relates to a compound microbial agent for preventing and treating tomato verticillium wilt, a preparation method and application thereof. Background technique [0002] Tomato is a herbaceous plant in the Solanaceae tomato genus, which has the value of food and medicine. In recent years, with the continuous expansion of the tomato cultivation area, coupled with successive plantings, it is difficult to fall stubble, which has aggravated the disease of tomato Verticillium wilt year by year, resulting in a significant reduction in yield in diseased fields, and seriously reduced the quality of tomato fruits and made them lose their commerciality. Verticillium wilt has become one of the most important diseases in facility tomato, and it is a bottleneck restricting tomato production and commercialization. Verticillium wilt is a soil-borne vascular disease caused by Verticillium dahliaKieb. It mo...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01N63/02A01P3/00C12R1/07C12R1/125
CPCA01N63/10B09C1/10C12N1/20C12N1/205C12R2001/07C12R2001/125
Inventor 孙海远赵钢勇顾欣燕刘亚利
Owner 天津坤禾生物科技集团股份有限公司
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