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Kit for diagnosis and detection of total protein by using potassium hydroxide method

A diagnostic kit and potassium hydroxide technology, which is applied in the field of clinical in vitro detection reagents, can solve the problems that biuret reagents cannot eliminate the interference of high fat and dextran, and achieve good reagent stability, high precision, and good measurement accuracy Effect

Inactive Publication Date: 2017-12-29
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a total protein detection and diagnosis kit by potassium hydroxide method, which effectively solves the problem that biuret reagents cannot eliminate the interference caused by high fat and dextran, and provides new information for the improvement of Doumas methodology

Method used

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  • Kit for diagnosis and detection of total protein by using potassium hydroxide method
  • Kit for diagnosis and detection of total protein by using potassium hydroxide method
  • Kit for diagnosis and detection of total protein by using potassium hydroxide method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In the specific implementation of the present invention, reagent R1 consists of:

[0041] Potassium iodide 3g / L

[0042] Sodium Potassium Tartrate 16g / L

[0043] Anhydrous copper sulfate 8g / L

[0044] Glycerol 3.0ml / L

[0045] Sodium azide 0.5g / L

[0046] Hydroxyethylidene diphosphonic acid 1ml / L

[0047] Reagent R2 consists of:

[0048] Potassium hydroxide 0.4M / L

[0049] Glycerol 3.0ml / L

[0050] Preservative 0.5g / L

[0051] Hydroxyethylidene diphosphonic acid 1ml / L

Embodiment 2

[0053] Reagent R1 consists of:

[0054] Potassium iodide 6g / L

[0055] Sodium Potassium Tartrate 12g / L

[0056] Anhydrous copper sulfate 8g / L

[0057] Glycerol 3.0ml / L

[0058] Sodium azide 0.5g / L

[0059] Hydroxyethylidene diphosphonic acid 1ml / L

[0060] Reagent R2 consists of:

[0061] Potassium hydroxide 0.4M / L

[0062] Glycerol 3.0ml / L

[0063] Sodium azide 0.5g / L

[0064] Hydroxyethylidene diphosphonic acid 1ml / L

Embodiment 3

[0066] Reagent R1 consists of:

[0067] Potassium iodide 6g / L

[0068] Sodium Potassium Tartrate 8g / L

[0069] Anhydrous copper sulfate 8g / L

[0070] Glycerol 3.0ml / L

[0071] Sodium azide 0.5g / L

[0072] Hydroxyethylidene diphosphonic acid 1ml / L

[0073] Reagent R2 consists of:

[0074] Potassium hydroxide 0.2mol / L

[0075] Glycerol 3.0ml / L

[0076] Sodium azide 0.5g / L

[0077] Hydroxyethylidene diphosphonic acid 1ml / L

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Abstract

The invention relates to the field of protein detection, mainly to a protein detection reagent. In the reagent R1, K<+> can effectively eliminate interference caused by high fat and dextran, so a biuret reagent is further improved in methodology; chelation between tartrate and potassium iodide enables effective substances to be stable for a long time; and 1-hydroxyethylidene-1,1-diphosphonic acid can guarantee quality stability of certain liquid, so the accuracy of detection results can be effectively guaranteed.

Description

technical field [0001] The kit of the invention relates to the technical field of clinical in vitro detection reagents, in particular to detecting and diagnosing the content of total protein in serum by a potassium hydroxide method. Background technique [0002] Serum proteins are carriers of fatty acids in the blood. Fatty acids are important to the body in two ways: they are the main building blocks of lipids, which make up all the biofilms around and within cells, and they are an inexhaustible source of energy. Our body has a storehouse of fatty acids - fat. When the body needs energy or building materials, fat cells release fatty acids into the blood, where they are picked up by serum proteins and transported to where they are needed. Therefore, it can be seen that serum protein plays an important role in the body. [0003] The biuret reagent test method is a long-standing clinical method for detecting serum proteins, especially the improved Doumas method, which is ad...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 甘宜梧李建营包兴艳谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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