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A specific marker for exogenous radish fragments and its preparation method and application

A specific marker and radish technology, which is applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as unstable inheritance, achieve genetic stability, avoid selfing decline, and eliminate biological confounding effects

Active Publication Date: 2021-04-06
湖南省作物研究所
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Problems solved by technology

For example, Li Xufeng reported that the restorer gene was introduced from the radish var. indigo to rapeseed, but the restorative genes of the obtained materials were all on the episomes, but could not be stably inherited; After crossing with a sterile line, the F 1 In order to genetically improve R2000, the recovered individual plants with changed markers were screened for radiation mutagenesis, but the recovered sources and molecular markers used were all from foreign research.
There is no self-developed molecular marker in our country that can be used for ogura-CMS restorer line breeding

Method used

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  • A specific marker for exogenous radish fragments and its preparation method and application
  • A specific marker for exogenous radish fragments and its preparation method and application
  • A specific marker for exogenous radish fragments and its preparation method and application

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Embodiment 1

[0053] The breeding method of the cytoplasmic sterile restorer line CLR095 of Brassica napus radish in the embodiment of the present invention is shown in the attached figure 1 As shown, it specifically includes the following steps.

[0054] 1. Development of specific molecular markers and linkage markers of radish cytoplasmic male sterility restorer gene

[0055] 1.1 Implementation materials

[0056] Brassica oleracea radish sterility recovery material CLR650 (it is radish male sterile cytoplasm, leaf edge is zigzag, leaf tip is sharper, leaf color is dark green, siliques are thick and short, see literature: Chen Weijiang et al., Brassica oleracea radish Creation of cytoplasmic male sterility restoration material, Agricultural Sciences of China, 2012, 45(8): 1465-1474), the material is a hybrid of Raphanobrassica (AACCRR, 2n=56) and Brassica napus through grafting technology Breeding, the number of chromosomes is between 38-40. Its cytoplasm is radish male sterile cytoplas...

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Abstract

The present invention relates to a preparation method and application of a specific marker for exogenous radish fragments of Brassica napus and a specific marker for exogenous radish fragments, and compares the whole genome resequencing data of CLR650, a cytoplasmic sterility recovery material of Brassica napus and radish genome, with the draft genome information of radish , developed the specific molecular markers CLR9‑1~CLR9‑20 of exogenous radish fragments. Through cytoplasmic sterility recovery material CLR650, the target individual plant of the backcross population was obtained to obtain BC 4 Separation of groups; the BC 4 For the fertile plants in the population, the mixed method is used for multi-generation mixed selection until a mixed segregation population with a fertile plant proportion exceeding 75% is obtained; finally, homozygous stability is achieved through multiple generations of selfing, and the cytoplasmic heterogeneity of Brassica napus radish is obtained. Restoration Department. The invention overcomes the obstacles in the existing method that the heritability of the restorer gene is seriously low and the homozygous stability of the character is difficult.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding and the field of molecular biology, and in particular relates to a specific marker for exogenous radish segments and a preparation method for specific markers for exogenous radish segments, and also relates to the application of specific markers for exogenous radish segments in the breeding of Brassica napus . Background technique [0002] Rapeseed has obvious heterosis, and is the oil crop with the most potential to cope with the shortage of edible vegetable oil and develop renewable bioenergy at home and abroad. Radish cytoplasmic male sterility (Ogu CMS) is an ideal type of sterility for the use of heterosis in Brassica napus. It is a cytoplasmic male sterility system established by Ogura, Japan in 1968, which found the source of sterility in radish. Because the sterility of the sterile system is very stable and thorough, it has very important value in the utilization of heterosis in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/10C12Q1/6895A01H1/02A01H1/04
Inventor 王同华郭一鸣涂金星陈卫江李莓范连益曲亮惠荣奎
Owner 湖南省作物研究所
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