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Prognostic marker of gliomas circ8: 127890589|127890998 and application

A glioma and marker technology, which is applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problems of poor treatment effect, poor prognosis, and unoptimistic postoperative survival rate

Inactive Publication Date: 2018-01-16
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical
At present, the diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the postoperative survival rate is not optimistic

Method used

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  • Prognostic marker of gliomas circ8: 127890589|127890998 and application
  • Prognostic marker of gliomas circ8: 127890589|127890998 and application
  • Prognostic marker of gliomas circ8: 127890589|127890998 and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of reagents for detecting expression of circRNA circ8:127890589|127890998 Kit for preparing prognosis of glioma patients (50 reactions)

[0019] 1. RNA stabilization solution 50ml

[0020] 2. Isopropanol 100ml

[0021] 3. Chloroform 100ml

[0022] 4. Trizol 50ml

[0023] 5. Enzyme-free water 10ml

[0024] 6.1 μM random reverse transcription primer 50 μl

[0025] 7.5× reverse transcription buffer 200ml

[0026] 8. 10mM base triphosphate deoxynucleotides 100μl

[0027] 9.40U / μl RNase inhibitor 500μl

[0028] 10.200U / μl MMLV reverse transcriptase 50μl

[0029] 11.Premix Ex Taq 50μl

[0030] 12.10μM circRNA circ8:127890589|127890998 real-time fluorescence quantitative PCR specific primer 30μl

[0031] circRNA circ8:127890589|127890998 Forward primer: 5'-

[0032] TTCCTTTGGGTCTCCCCTAT-3',

[0033] circRNA circ8:127890589|127890998 Reverse primer: 5'-

[0034] TGTAAATCCATCAGGCTCAG-3';

[0035] 13.10 μM GAPDH specific primer 30 μl

[0036] T...

Embodiment 2

[0038] Example 2 Detection of tissue sample circRNA circ8:127890589|127890998

[0039] 1. Collect the glioma tissue to be tested, put it into a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0040] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add chloroform 200μl / ml Trizol to the Tube, shake it by hand for 15-30s, place on ice for 5min, centrifuge at 12000rpm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add pre-cooled isopropanol 0.5ml / ml Trizol Mix well, put it in a refrigerator at -20°C for 20min, cent...

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Abstract

The invention discloses a prognostic marker of gliomas circ8: 127890589|127890998 and application, namely circRNA circ8: 127890589|127890998 reagents of detecting brain tissue sources for preparing the prognostic marker of gliomas patients. Researchers find that an expression level of the circRNA circ8: 127890589|127890998 is significantly downregulated in brain tissues of the glioma patients (P less than 0.0001). Patients with higher expression levels of circRNA circ8: 127890589|127890998 in gliomas have a higher postoperative survival rate (P equal to 0.086). Therefore, the prognostic markerof the gliomas circ8: 127890589|127890998 and the application can determine prognosis of the glioma patients by detecting the expression level of circRNA circ8: 127890589|127890998 in the glioma tissues of the glioma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker for glioma prognosis, the application of a reagent for detecting the marker to prepare a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most common malignant neuroepithelial cell tumor in the brain, accounting for 40.49% of intracranial tumors, and is one of the most common brain tumors in adults. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical. At present, the diagnosis of glioma is still...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 李沛瑶武明花汤颖刘庆刘强刘涛刘长红
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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