A strain of Enterobacter ludwig sxz-n5 producing huperzine A and huperzine B and its use

Abstract

The invention provides an enterobacter ludwigii SXZ-N5 strain producing huperzine A and huperzine B and application thereof. The strain is capable of producing huperzine A and huperzine B. The strainis named as SXZ-N5 and belongs to enterobacter ludwigii. The strain is collected in China Center for Type Culture Collection Center. The address is Wuhan University, Wuhan, China. The postal code is 430072. The strain has a collection date of September 14, 2017, and a collection number of CCTCC NO: M2017511. The 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The enterobacter ludwigii SXZ-N5 strain disclosed by the invention can be used for fermenting to prepare the huperzine A and huperzine B. Microbial fermentation conditions are as follows: a culture medium formula: 4.0g of yeast powder, 8.0g / L of peptone, 7.0g / L of sodium chloride, 9.0g / L of glucose, the pH value of 6.0; as well as culture conditions comprising 28 DEG C and 200 revolutions per minute, and fermented culture isperformed by 10 days.

Description

technical field [0001] The invention relates to an Enterobacter ludwig strain, which can produce huperzine A and huperzine B, and belongs to the technical field of microorganisms. Background technique [0002] At present, the research on plant endophytes has been paid more and more attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to discover new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that plant endophytes can produce the same or similar active substances and precursors as host plants. This discovery directly promotes the extensive research on plant endophytes, especially medicinal plant endophytes. With people's gradual research on endophytes of medicinal plants, using endophytes that can metabolize and produce corresponding...

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Problems solved by technology

However, due to the scarcity of Huperzia serrata plant resources, slow growth, and low content of huperzine A, only relying on the extraction of huperzine A from Huperzia serrata cannot meet the market demand. However, the activity of chemically synthesized huperzine A is far lower. It is better than natural huperzine A, and the cost of obtaining highly optically active huperzine A is relatively high

At present, there are some relevant reports in the field of using Huperzia serrata endophytes for microbial fermentation to produce huperzine A, but the types and yields of these strains still cannot meet the market demand for huperzine A

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