A strain of Enterobacter ludwig sxz-n5 producing huperzine A and huperzine B and its use

A technology of Enterobacter ludwigii and SXZ-N5, applied in the field of microorganisms, can solve the problems of lack of resources, high cost of Huperzine A, strain types and yields that cannot meet Huperzine A, etc., and achieves low fermentation cost. Effect

Active Publication Date: 2019-09-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

However, due to the scarcity of Huperzia serrata plant resources, slow growth, and low content of huperzine A, only relying on the extraction of huperzine A from Huperzia serrata cannot meet the market demand. However, the activity of chemically synthesized huperzine A is far lower. It is better than natural huperzine A, and the cost of obtaining highly optically active huperzine A is relatively high
At present, there are some relevant reports in the field of using Huperzia serrata endophytes for microbial fermentation to produce huperzine A, but the types and yields of these strains still cannot meet the market demand for huperzine A

Method used

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  • A strain of Enterobacter ludwig sxz-n5 producing huperzine A and huperzine B and its use
  • A strain of Enterobacter ludwig sxz-n5 producing huperzine A and huperzine B and its use
  • A strain of Enterobacter ludwig sxz-n5 producing huperzine A and huperzine B and its use

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0013] Isolation and cultivation of strains

[0014] In this example, the Huperzia serrata material was rinsed with clean water, and then ultrasonicated with clean water for 10 minutes to separate the leaves and stems and cut into small sections of about 4 cm, soaked in 0.1% mercury liter for 4 minutes, and then soaked in 70% ethanol for 2 minutes, Soak in 5% sodium hypochlorite for 5 minutes, then rinse with sterile water several times, grind it into a homogenate under sterile conditions, take 200 μl of the homogenate on each PDA plate without antibiotics, and culture at 30°C for 1 -2d. During the culture process, 20 colonies were randomly selected according to the growth of the bacteria, connected to a new LA medium, and separated and passaged through multiple plate streaking metho...

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Abstract

The invention provides an enterobacter ludwigii SXZ-N5 strain producing huperzine A and huperzine B and application thereof. The strain is capable of producing huperzine A and huperzine B. The strainis named as SXZ-N5 and belongs to enterobacter ludwigii. The strain is collected in China Center for Type Culture Collection Center. The address is Wuhan University, Wuhan, China. The postal code is 430072. The strain has a collection date of September 14, 2017, and a collection number of CCTCC NO: M2017511. The 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The enterobacter ludwigii SXZ-N5 strain disclosed by the invention can be used for fermenting to prepare the huperzine A and huperzine B. Microbial fermentation conditions are as follows: a culture medium formula: 4.0g of yeast powder, 8.0g / L of peptone, 7.0g / L of sodium chloride, 9.0g / L of glucose, the pH value of 6.0; as well as culture conditions comprising 28 DEG C and 200 revolutions per minute, and fermented culture isperformed by 10 days.

Description

technical field [0001] The invention relates to an Enterobacter ludwig strain, which can produce huperzine A and huperzine B, and belongs to the technical field of microorganisms. Background technique [0002] At present, the research on plant endophytes has been paid more and more attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to discover new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that plant endophytes can produce the same or similar active substances and precursors as host plants. This discovery directly promotes the extensive research on plant endophytes, especially medicinal plant endophytes. With people's gradual research on endophytes of medicinal plants, using endophytes that can metabolize and produce corresponding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/12C12P17/18C12R1/01
Inventor 张鹏张学智宋发军刘盼李浩东黄冰王红莹
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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