Microfluidic Chemiluminescent Detection System for Magnetic Microparticles for the Detection of Cardiac Enzyme Series

Abstract

The invention discloses a microfluidic chemiluminescence detecting system for detecting magnetic particles of myocardial enzyme series. The microfluidic chemiluminescence detecting system comprises abase plate and an upper chip. The upper chip comprises a sample adding area and three microfluidic reaction detecting channels, the sample adding area is located at the center of the upper chip, and the microfluidic reaction detecting channels are communicated with the sample adding area. The microfluidic reaction detecting channels include a cardiac troponin I microfluidic reaction detecting channel, a myohemoglobin microfluidic reaction detecting channel and a creatine jubase-isozyme microfluidic reaction detecting channel. During application, the base plate is arranged on the lower side ofthe upper chip. Magnets, namely permanent magnets or electromagnets, are arranged at positions of magnetic particle coating areas, corresponding to the microfluidic reaction detecting channels, of thebase plate.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to a microfluidic chemiluminescent detection system for detecting magnetic particles of myocardial enzyme series. Background technique [0002] In recent years, the field of bioanalysis technology has developed rapidly, and many important research directions have emerged. Microfluidic chip analysis technology is one of the most active ones, and has received extensive attention in both scientific research and application fields. As a new type of analysis and detection platform, microfluidic chips have the advantages of high throughput, integration, portability, easy operation, and low cost, and have been widely used in many fields. [0003] The current chemiluminescence detection is suitable for serum, and the sample needs to be centrifuged to obtain serum during the detection process, which is complex, tedious and time-consuming. Lack of sufficient elution to eliminate i...

AI Technical Summary

Problems solved by technology

Cardiac troponin I (cTnI), myoglobin (MYO), and creatine kinase isoenzyme (CK-MB) belong to the detection series of myocardial enzymes. Patients often need to detect a series of items in myocardial enzymes. In the existing technology Among them, the test cards of each manufacturer are usually individually packaged for a single item. According to the current method, the laboratory physician needs to perform the test operation item by item, the workload is heavy, and the testing process is repetitive and cumbersome. Treatment time, but also a waste of resources

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