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Composite amplification detection kit containing 35 human Y-STR gene loci

A detection kit and multiple amplification technology, applied in the field of molecular genetics, can solve the problems of complex operation and unstable performance, and achieve the effect of simple operation, high polymorphism and stable performance

Inactive Publication Date: 2018-02-02
安徽安龙基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects of unstable performance and complicated operation in the prior art, and provide a compound amplification detection kit containing 35 human Y-STR bases to solve the above problems

Method used

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  • Composite amplification detection kit containing 35 human Y-STR gene loci
  • Composite amplification detection kit containing 35 human Y-STR gene loci
  • Composite amplification detection kit containing 35 human Y-STR gene loci

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Screening of Y-STR 35 loci

[0032] 通过对DYS19、GATA_H4、DYS388、DYS390、DYS391、DYS392、DYS393、DYS389I、DYS389II、DYS437、DYS439、DYS458、DYS460、DYS468、DYS481、DYS385a / b、 DYS456、DYS444、DYS447、DYS438、DYS448、DYS387、DYS517、 Study on the polymorphism and mutation rate of 39 Y chromosome STR loci including DYS518, DYS557 a / b, DYS576, DYS570, DYS449, DYS527a / b, SYS533, DYS549, DYS635, DYS612, DYS643, DYS626, DYS627, and finally A total of 35 Y-STR loci were screened out, and the corresponding information table for each locus is as follows:

[0033]

[0034]

Embodiment 2

[0036] Establishment of five-color fluorescence system

[0037] The specific process of using fluorescence detection technology in forensic laboratories is as follows: laser bombards the fluorescent group (dye) connected to the end of DNA, and the fluorescent group emits light with lower energy after absorbing ability, and uses a filter to collect the emitted light in a specific wavelength range. Photomultiplier tubes or electrically coupled devices are used to collect and amplify the signals from fluorescent groups, and convert them into electrical signals, and finally generate the peak map of capillary electrophoresis.

Embodiment 3

[0039] 35Y-STR loci arrangement

[0040] The first group: DYS458, DYS460, DYS437, DYS439, DYS481, DYS385a / b, DYS557 a / b;

[0041] The second group: DYS456, DYS391, DYS390, DYS635, DYS19, GATA_H4, DYS447;

[0042] The third group: DYS438, DYS389I, DYS448, DYS389II, DYS387, DYS518;

[0043] The fourth group: DYS393, DYS576, DYS570, DYS392, DYS449, DYS527a / b;

[0044] The fifth group: DYS444, DYS388, DYS643, SYS533, DYS549, DYS627;

[0045] The 5' ends of the five sets of primers were labeled with blue FAM, green VIC, yellow NED, red PET, and pink fluorescein HEX, respectively.

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PUM

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Abstract

The invention discloses a composite amplification detection kit containing 35 human Y-STR gene loci. The composite amplification detection kit is characterized in that the gene loci include DYS458, DYS460, DYS437, DYS439, DYS481, DYS385a / b, DYS557a / b, DYS456, DYS391, DYS390, DYS635, DYS19, GATA_H4, DYS447, DYS438, DYS389I, DYS448, DYS389II, DYS387, DYS518, DYS393, DYS576, DYS570, DYS392, DYS449, DYS527a / b, DYS444, DYS388, DYS643, SYS533, DYS549 and DYS627. The composite amplification detection kit is high in polymorphism, stable in performance, simple to operate, and the like.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a composite amplification detection kit containing 35 human Y-STR-based loci. Background technique [0002] The human Y chromosome belongs to the sex chromosome, which is unique to normal males. It does not undergo recombination during the genetic process, and its sequence structure is passed on from the parent to the offspring unchanged except for mutations. The Y chromosomes of the same paternal population are theoretically the same. This characteristic of the Y chromosome makes it possible to identify the mixed samples of men and women extracted from cases of sexual assault and injury in actual case handling. Since the genetic information of the Y chromosome of a male individual is not affected by the female component, the Y chromosome For a long time, it has been used as an important research object for forensic evidence inspection. [0003] Short tandem repeat sequence (ST...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/16C12Q2525/151C12Q2563/107
Inventor 韦玉军李航李静
Owner 安徽安龙基因科技有限公司
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