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Manufacturing process for polysaccharide beads

A technology for polysaccharide beads and polysaccharide, which is applied in the field of preparing polysaccharide beads, cross-linked polysaccharide beads, and preparing polysaccharide beads by reversed-phase suspension technology, can solve problems such as elimination, and achieve the effect of good pore structure and mechanical properties

Active Publication Date: 2018-02-06
CYTIVA BIOPROCESS R&D AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The issue in this paper is that the current large-scale use of halocarbons and aromatics is being phased out for environmental reasons

Method used

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  • Manufacturing process for polysaccharide beads
  • Manufacturing process for polysaccharide beads
  • Manufacturing process for polysaccharide beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Emulsification method (reference example uses 1,2-dichloroethane)

[0044] 165 g of dextran (Mw 150-250 kD) was dissolved in 390 ml of distilled water while 22 ml of 50% sodium hydroxide (NaOH) was added under stirring. 0.8 g sodium borohydride (NaBH 4 ) was added to the solution.

[0045] 21 g of cellulose acetate butyrate (CAB) were added to a 1000 ml glass reactor with an overhead stirrer. Start the stirrer and add 350 ml 1,2-dichloroethane (EDC) (so the CAB concentration is 0.060 g / ml EDC). The solution was stirred until the CAB was dissolved. The aqueous phase was then added to the oil phase with stirring at 50°C, and stirring continued until a suitable droplet size was achieved, as judged from microscopic examination of a small sample drawn. When the proper size was obtained, the emulsion was immediately stabilized by adding 22.4 ml of epichlorohydrin (ECH) to cross-link the dextran and thus solidify the droplets. 20 minutes after the addition of ECH, 50 ml o...

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Abstract

The invention discloses a method of manufacturing polysaccharide beads, comprising the steps of: i) providing a water phase comprising an aqueous solution of a polysaccharide; ii) providing an oil phase comprising at least one water-immiscible organic solvent and at least one oil-soluble emulsifier; iii) emulsifying the water phase in the oil phase to form a water-in-oil (w / o) emulsion; and iv) inducing solidification of the water phase in the w / o emulsion, wherein the organic solvent is an aliphatic or alicyclic ketone or ether.

Description

[0001] Technical Field of the Invention [0002] The present invention relates to polysaccharide beads, and more particularly to the preparation of polysaccharide beads by the reverse phase suspension technique. The invention also relates to crosslinked polysaccharide beads and to the use of the beads for separation purposes. Background of the invention [0003] Cross-linked polysaccharide beads are commonly used as stationary phases for chromatographic separations of proteins and other biomolecules. Such beads were introduced in the early 1960's (see eg US3208994, which is hereby incorporated by reference in its entirety), primarily for laboratory separation purposes. Since then their use has greatly increased, and cross-linked polysaccharide beads are now routinely used in large-scale preparation processes for the separation of many biopharmaceuticals such as monoclonal antibodies, plasma components, insulin and various recombinant proteins. [0004] The most common method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/16C08J3/24C08L5/02C07K1/18C12N5/00
CPCC07K1/18C08J3/16C08J3/24C12N5/0075C08J2305/02C08B37/0021C08L5/02B01J13/14C07K1/22C08L2203/02C08L2205/02C12N2531/00C12N2533/70C12N2533/78C12N2537/10
Inventor P.E.埃米斯森S.K.M.林德伯格J.维纳斯森J.古斯塔夫斯森A.胡赖诺维茨
Owner CYTIVA BIOPROCESS R&D AB
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