Extraction method of cucurbitane-type triterpene compound and its anti-aging medicinal application

A cucurbitane-type and compound technology, applied in the field of drug extraction, can solve the problems of little drug, and achieve the effects of obvious activity, high safety, and prolongation of replicative lifespan

Active Publication Date: 2020-05-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scientists have done a lot of work on aging and anti-aging research, but very few drugs have been clinically tested and marketed

Method used

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  • Extraction method of cucurbitane-type triterpene compound and its anti-aging medicinal application
  • Extraction method of cucurbitane-type triterpene compound and its anti-aging medicinal application
  • Extraction method of cucurbitane-type triterpene compound and its anti-aging medicinal application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Extract cucurbitane type triterpenoids in bitter melon fruit

[0040] A method for extracting cucurbitane-type triterpenoids from balsam pear fruits, comprising the following steps:

[0041] (1) After pulverizing 1.5kg (dry weight) of bitter gourd (Momordica charantia), extract it with 5L industrial grade methanol, place it in a shaker for 3 days with shaking at room temperature, vacuum filter, take the filtrate, and concentrate under reduced pressure to obtain Methanol extract 224g.

[0042] (2) Alternately use ethyl acetate and water to dissolve the sample of the methanol extract obtained in (1) and transfer it to a separatory funnel for distribution. After standing overnight, spin the ethyl acetate layer and the water layer to obtain ethyl acetate Layer sample 30g, water layer sample 170g.

[0043] (3) The sample of the ethyl acetate layer is separated by a silica gel open column (silica gel 200-300 mesh), and the elution system is n-hexane:acetone=99...

Embodiment 2

[0067] Example 2: Anti-aging activity analysis of compounds 1-11

[0068] The biological models currently used for anti-aging research mainly include mice, nematodes, fruit flies and yeast. In this application example, Saccharomyces cerevisiae was selected as a biological model to screen anti-aging active compounds. Yeast is a eukaryotic single-celled organism with a small genome, short life cycle, and low cost of culture. It is currently a commonly used aging model organism. Resveratol is a polyhydric phenol found in red wine, which has shown anti-aging and anti-aging-related disease activities in various animal models.

[0069] The analytical method includes the following steps:

[0070] (1) Take out the K6001 yeast strain from the -30°C refrigerator, wash it three times with PBS, 5ml each time, and remove the glycerol therein. Finally, 1 ml of PBS was added, blown to make it suspended, and then added to 5 ml of liquid medium (1% yeast powder, 2% peptone, 3% galactose). ...

Embodiment 3

[0080] Example 3: Activity Analysis of Compound 10 Improving Yeast Survival Rate

[0081] To test whether compound 10 can improve the survival rate of yeast, the test method is as follows:

[0082] (1) Wash the wild-type yeast BY4741 stored at -30°C with 5ml PBS three times to remove the glycerol; add 1ml sterile water, blow it to suspend it, add it to 5ml glucose medium (1% yeast powder, 2% peptone, 2% glucose); put it into a shaker, and shake (160r / min) at 28°C for 24 hours to restore its growth ability.

[0083] (2) Inoculate BY4741 into 25mL new glucose medium, adjust OD 600 The value is 0.1, and then incubated with a certain concentration of compound 10, a negative control substance, and a positive control substance (10 μM resveratrol) for 12 hours.

[0084] (3) After incubating BY4741 with samples and controls for 12 hours, about 200 cells from each group were coated with or without 5mM H 2 o 2 Glucose solid medium; calculate the survival rate of yeast cells after 2 ...

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Abstract

The invention provides a cucuribitane triterpene compound 1-11 and an extraction method thereof. The extraction method comprises the following steps: grinding bitter gourd fruit into a powder, puttingthe powder into methyl alcohol for extracting, extracting an extract with ethyl acetate and water so as to obtain crude extracts of a water layer and an ester layer; carrying out separation purification on the crude extracts respectively so as to obtain the compound 1-11. The extraction method provided by the invention is simple and practicable in operation and high in yield. According to the cucuribitane triterpene compound provided by the invention, in an anti-aging in vitro screening model, the replicability service lives of yeast cells are obviously prolonged. The compound can be appliedto preparing anti-aging medicines and health products. A basis is provided for research and development and basic research of anti-aging new drugs, and the compound has an important practical significance. The structural formula of the cucuribitane triterpene compound is as shown in the description.

Description

technical field [0001] The invention belongs to the field of medicine extraction, and relates to a method for extracting cucurbitane-type triterpene compounds, in particular to a method for extracting picrocucurbitane-type triterpene compounds and its application in preparing anti-aging products. Background technique [0002] With the development of society, the improvement of living and medical standards, the life expectancy of human beings is extended, resulting in an increase in the elderly population. According to the latest United Nations statistics on the world population, in 2015, the world's elderly population reached 901 million, accounting for 12% of the total population, and this proportion will continue to increase at a rate of 3.26% per year. It is estimated that by 2050, this number It will reach 2.1 billion, and the elderly population in major regions of the world will account for a quarter. The world has entered an aging society. China's population is aging ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00C07J17/00A23L33/11A61K31/58A61K31/585A61P39/00
CPCA23L33/11A23V2002/00C07J17/005C07J71/0005A23V2200/302A23V2250/21
Inventor 戚建华曹雪丽孙玉娟林燕飞向兰
Owner ZHEJIANG UNIV
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