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A method for high-efficiency cutting and rapid propagation of blue flower dan

A technology of blue flower dan and cuttings, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of unfavorable cost, easy to cause pollution, control, etc., achieve simple and easy operation, reduce pollution, reduce cost effect

Active Publication Date: 2019-12-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current rapid propagation system usually requires a large amount of exogenous hormones, which is not conducive to cost control and easy to cause pollution for large-scale breeding

Method used

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  • A method for high-efficiency cutting and rapid propagation of blue flower dan
  • A method for high-efficiency cutting and rapid propagation of blue flower dan
  • A method for high-efficiency cutting and rapid propagation of blue flower dan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 cutting propagation in advance

[0031] (1) Aseptic sowing and germination

[0032] Aseptic sowing of seeds: Harvest mature blueflower fruits from October to December and dry them in a constant temperature hair dryer at 40°C. After they are completely dried and most of the seeds burst, they should be manually rubbed properly to make the seeds completely crack. Collect and record the number of seeds, put them into kraft paper bags and mark them, and store them in a dark and dry seed bank for later use. Select mature, plump and pest-free blue flower seeds, soak the seeds in tap water at 28°C for 0-2 days, soak them in washing powder water for 0.5 hours, and then rinse them with running water for 2 hours. 2 Soak for single-factor complete random disinfection treatment, and then inoculate on the medium described in Tables 2 and 3 for two-factor and three-level aseptic germination treatment, inoculate 3 seeds in each bottle, and repeat 3 times for each treatmen...

Embodiment 2

[0051] After the cutting propagation in advance of embodiment 1, treat that the rooted scion grows to more than 10cm, can cut the scion for the second time, and the length is no more than 1 / 2 of the original length. Insert the newly cut shoots as the second-generation scion (abbreviated as F2) into MS medium containing NAA (0.51.01.5mg / L), IBA (0.51.01.5mg / L), and hormone-free . The result is as follows:

[0052] Table 5 Screening table of inducing hormones for rooting of aseptic cuttings of Lanhuadan F2

[0053]

[0054] It can be seen from Table 5 that the rooting condition of the second-generation scion of Lanhuadan in the hormone blank medium has reached or exceeded that of the medium using IBA. Compared with the blank medium and IBA medium, the medium containing NAA could not effectively induce the rooting of F2 scions, and a large number of calluses would appear.

[0055] Will contain 1.0mg·L -1 NAA, 0.5mg·L -1 From the plants obtained on IBA and blank medium, th...

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Abstract

The invention provides a method for efficiently cutting and rapidly propagating plumbago auriculate. The method comprises the following steps of cutting off epicormic branches of plumbago auriculate offsprings obtained through cutting propagation in advance as cions, cutting and cultivating the cions into a culture medium without exogenous hormone; the culture medium without exogenous hormone is an MS culture medium added with saccharose and agar. The in-advance cutting propagation method comprises the steps of cutting off epicormic branches of plumbago auriculate seedlings as cions, and cutting and cultivating the cions in the MS culture medium added with a plant growth regulator, the saccharose and the agar, wherein the plant growth regulator is NAA and / or IBA. According to the method provided by the invention, an effect on cutting and rapidly propagating the plumbago auriculate can be achieved without the need for adding any exogenous hormone, the cost is greatly reduced, and the corresponding effect is reduced; the rooting time is early, the rotting rate is high, the growth condition is good, and the method is applicable to be popularized and applied; the method provided by theinvention is simple and easy to operate, and is easy to quickly obtain plumbago auriculate commercial seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, relates to the technical field of blue flower dan breeding, and in particular relates to a method for efficient cutting and rapid propagation of blue flower dan. Background technique [0002] As a multifunctional garden ornamental medicinal plant, blue flower dan has a very long flowering period (up to half a year), elegant and unique flower color, strong sprouting ability and stress resistance, simple and extensive management, less pests and diseases, high temperature resistance, Adult plants have large crowns and are resistant to pruning. They have been introduced, cultivated and applied more and more, and have gradually become the new favorites in modern garden plant landscaping; plumbagin accumulated in large quantities in their roots has anti-cancer, anti-oxidation, Various pharmacological effects such as antibacterial, radiosensitization, and contraception have attracted more a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高素萍雷霆张硕李文骥朱原闻金燕
Owner SICHUAN AGRI UNIV