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Breeding method for pure line of head shape character of artificially induced gynogenesis carassius auratus

A technology of gynogenesis and artificial induction, applied in fish farming, application, climate change adaptation, etc.

Active Publication Date: 2018-02-16
福州春园鲤水族有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many strains that can stably inherit the head shape traits of goldfish breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Artificial ejaculation

[0027] Adopt blue-grey gonad-mature male carp as broodstock. Firstly, 2 μg of luteinizing hormone-releasing hormone (LHRH-A1) was injected into each fish; then 500-800 U of human chorionic gonadotropin (HCG) was injected per kg of fish to induce ejaculation, and semen was collected about 10 hours later. The collected male carp semen was diluted with 60-fold Hank's solution to obtain a sperm dilution.

[0028] 2. Sperm inactivation

[0029] Use the Atmospheric Room Temperature Plasma Breeding Apparatus (ARTP) to inactivate sperm in batches to obtain inactivated sperm fluid; add 5 μL 0.6M mannitol and 10 μL Hank's solution to each 20 μL inactivated sperm fluid after treatment , to obtain a dilution of the inactivated sperm, and store it in the dark at -2 to 4°C for later use. Multiple batches of processed inactivated sperm dilutions were combined and stored for future use.

[0030] Described sperm inactivation process, its condition is: spe...

Embodiment 2

[0044] 1. Artificial ejaculation

[0045] Adopt blue-grey gonad-mature male carp as broodstock. Firstly, 3 μg of luteinizing hormone-releasing hormone (LHRH-A1) was injected per fish; then 500 U of human chorionic gonadotropin (HCG) was injected per kg of fish to induce semen, and the semen was collected about 10 hours later. The collected male carp semen was diluted with 70-fold Hank's solution to obtain a sperm dilution.

[0046] 2. Sperm inactivation

[0047] Use the Atmospheric Room Temperature Plasma Breeding Apparatus (ARTP) to inactivate sperm in batches to obtain inactivated sperm fluid; add 5 μL 0.6M mannitol and 10 μL Hank's solution to each 20 μL inactivated sperm fluid after treatment , to obtain a dilution of the inactivated sperm, and store it in the dark at -2 to 4°C for later use. Multiple batches of processed inactivated sperm dilutions were combined and stored.

[0048] Described sperm inactivation process, its condition is: sperm diluent loading sample v...

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PUM

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Abstract

The invention relates to a breeding method for a pure line of a head shape character of artificially induced gynogenesis carassius auratus. The method comprises the following steps: collecting semen from caesious male carp taken as parent fish, diluting the semen, and preforming sperm inactivation treatment by using a breeding instrument adopting normal-pressure and room-temperature plasma; discharging female carassius auratus roe with the target head shape character into a porcelain basin, pouring inactivated sperms into the basin, and spreading zygotes on a fish nest; soaking the zygotes ina cytochalasin B solution for 20-30 minutes, performing incubating naturally for 50-100 days, and discarding caesious individual carassius auratus fries and gynogenesis carassius auratus fries with non-target head shape character to obtain the carassius auratus of generation F1; taking female carassius auratus of the generation F1 and male carp as parent fish, and repeating the steps to obtain thecarassius auratus pure line of generation F2 with the target head shape character. The carassius auratus pure line of generation F2 is obtained with the method and taken as the parent fish to be usedfor breeding production, and a large amount of carassius auratus with excellent ornamental head shape character can be bred to meet the requirements of people.

Description

technical field [0001] The invention belongs to the technical field of goldfish artificial breeding, and in particular relates to a method for artificially inducing gynogenetic goldfish pure breed breeding method for head shape traits. Background technique [0002] Artificially induced fish gynogenesis is of great significance in theoretical research and production practice. Its research involves almost all aquaculture fish, and it is widely used in the breeding and genetic improvement of fish species, the identification of sex determination mechanism, Gene mapping and establishment of pure lines, etc.; combined with directional selection and hybridization techniques, many strains with excellent traits such as fast growth, good body shape, strong fecundity and strong disease resistance have been bred, becoming the most active fish genetics and breeding research. one of the fields. The application of artificially induced fish gynogenesis as an ornamental fish is also active....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 黄镇陈多岑万周翊涛黄祖新
Owner 福州春园鲤水族有限公司
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